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Mertk 108928

Manufactured by R&D Systems
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MerTK (108928) is a recombinant human MER/MERTK protein. MER is a receptor tyrosine kinase that plays a role in the phagocytosis of apoptotic cells. The protein contains the extracellular domain of MER fused to the Fc region of human IgG1.

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Lab products found in correlation

Leucoperm, by Bio-Rad (2 mentions) Cd68 fa 11, by Bio-Rad (2 mentions) F4 80 bm8, by Thermo Fisher Scientific (2 mentions) Cd14 sa14 2, by BioLegend (2 mentions) Facs caliber, by BD (2 mentions) Facsdiva, by BD (2 mentions) Cellquest, by BD (2 mentions) Facscanto, by BD (2 mentions) Siglecf e50 2440, by BD (2 mentions)

2 protocols using mertk 108928

1

Purification and Immunophenotyping of BAL Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
BAL cells were purified by centrifugation on Percoll to remove surfactant and debris 31 (link). BAL cells or BMDMs were immunostained to detect CD115 (AFS98), F4/80 (BM8) (eBioscience), CD3 (145-2C11), CD11b (M1/70), CD11c HL3), CD16/32 (2.4G2), CD19 (1D3), CD64 (X54-5/7.1.1), CD131 (JORO50), Ly6G (1A8), CD45.1 (A20), CD45.2 (104), MHC class II (I-A/I-E) (M5/114.15.2), SiglecF (E50-2440) (BD Biosciences), CD14 (Sa14-2) (BioLegend), CD68 (FA-11) (AbD Serotec), and MerTK (108928) (R&D Systems), as previously described1 (link), evaluated by flow cytometry using a FACSCaliber or FACSCanto flow cytometers (both from BD Biosciences, San Jose, CA), and the results were analyzed using CellQuest, FACSDiva (BD Biosciences), or FlowJo software (Tree Star). For intracellular staining of CD68, Leucoperm (AbDSerotec) was used as directed by the manufacturer.
Suzuki T., Arumugam P., Sakagami T., Lachmann N., Chalk C., Sallese A., Abe S., Trapnell C., Carey B., Moritz T., Malik P., Lutzko C., Wood R.E, & Trapnell B.C. (2014). Pulmonary Macrophage Transplantation Therapy. Nature, 514(7523), 450-454.
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2

Purification and Immunophenotyping of BAL Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
BAL cells were purified by centrifugation on Percoll to remove surfactant and debris 31 (link). BAL cells or BMDMs were immunostained to detect CD115 (AFS98), F4/80 (BM8) (eBioscience), CD3 (145-2C11), CD11b (M1/70), CD11c HL3), CD16/32 (2.4G2), CD19 (1D3), CD64 (X54-5/7.1.1), CD131 (JORO50), Ly6G (1A8), CD45.1 (A20), CD45.2 (104), MHC class II (I-A/I-E) (M5/114.15.2), SiglecF (E50-2440) (BD Biosciences), CD14 (Sa14-2) (BioLegend), CD68 (FA-11) (AbD Serotec), and MerTK (108928) (R&D Systems), as previously described1 (link), evaluated by flow cytometry using a FACSCaliber or FACSCanto flow cytometers (both from BD Biosciences, San Jose, CA), and the results were analyzed using CellQuest, FACSDiva (BD Biosciences), or FlowJo software (Tree Star). For intracellular staining of CD68, Leucoperm (AbDSerotec) was used as directed by the manufacturer.
Suzuki T., Arumugam P., Sakagami T., Lachmann N., Chalk C., Sallese A., Abe S., Trapnell C., Carey B., Moritz T., Malik P., Lutzko C., Wood R.E, & Trapnell B.C. (2014). Pulmonary Macrophage Transplantation Therapy. Nature, 514(7523), 450-454.
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