PC12 cell viability was determined using a Cell Counting Kit-8 (CCK-8) cell viability assay (Dojindo Molecular Technologies, Inc., Kumamoto, Japan). PC12 cells were incubated in 96 well plates with 10,000 cells per well for cell viability analysis. Following the final 24 h incubation step of OGD/R or IPOC treatments, 20 µl CCK-8 solution was added to each well containing 200 µl culture medium. After incubating the cells at 37°C for 2 h, the absorbance at 450 nm (A450) was measured using a Multiskan FC microplate spectrophotometer (Thermo Fisher Scientific, Inc.). Cell viability (%) was calculated as: (A450 of test well / A450 of control well) × 100.
Cell counting kit 8 cck 8 cell viability assay
Manufactured by Dojindo Laboratories
Sourced in Japan
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay for the determination of cell viability. It utilizes a water-soluble tetrazolium salt to measure the number of viable cells. The assay can be performed in 96-well microplates, and the absorbance is measured using a spectrophotometer.
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3 protocols using cell counting kit 8 cck 8 cell viability assay
1
Quantifying PC12 Cell Viability
2
HUVEC Viability Assay with Lenvatinib
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HUVECs viability was determined using the cell counting kit-8 (CCK-8) cell viability assay (Dojindo Laboratories, Kumamoto, Japan). HUVECs (3×103 cells per well) were seeded in flat-bottomed 96-well plates. After incubation for 24 h, the cells were treated with lenvatinib at various concentrations and 60 ng/ml VEGFA165 (PeproTech Inc.) in serum-reduced (1%) RPMI 1640 medium for 72 h. The final concentration of dimethyl sulfoxide (DMSO, as vehicle) was ≤1%. Results were expressed as the mean ± S.E.M. of at least three independent proliferation assays. The dose-response curve and half maximal inhibitory concentration (IC50) values of lenvatinib were calculated busing the GraphPad Prism software (GraphPad Software Inc., San Diego, CA, USA).
3
Cell Proliferation Evaluation by CCK8 Assay
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Proliferation of SCC15 and SCC25 cells were evaluated by a Cell Counting Kit-8 (CCK8) cell viability assay (Dojindo Molecular Technologies, Inc.), according to the manufacturer's protocol. For the CCK-8 assay, 5×103 cells were seeded into 96-well plates. Following incubation at 37°C for 48 and 72 h, cell viability was measured at a wavelength of 450 nm using a spectrophotometric plate reader.
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