Total RNA was extracted as described above using Total RNA Purification Kit (Norgen-Biotek Corp.) according to the manufacturer's instructions. One hundred and fifty nanograms of total RNA was labeled and then hybridized to the Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip (Agilent Technologies). All microarray experiments were conducted at the Microarray Core Facility (Stem Cell Unit, King Saud University College of Medicine). Normalization and data analyses were conducted using GeneSpring GX software (Agilent Technologies). Pathway analysis were conducted using the Single Experiment Pathway analysis feature in GeneSpring 12.0 (Agilent Technologies) as described before.33 (link), 34 (link) Twofold cutoff with P<0.02 was used.
Agilent human sureprint g3 human ge 8 60 k v16 microarray chip
Manufactured by Agilent Technologies
Sourced in United States
The Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip is a high-density DNA microarray designed for gene expression analysis. It contains approximately 60,000 probes representing human genes and transcripts.
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Lab products found in correlation
3 protocols using agilent human sureprint g3 human ge 8 60 k v16 microarray chip
1
Microarray Analysis of Total RNA
2
Microarray Analysis of Total RNA
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Total RNA was extracted using PureLink RNA Mini Isolation Kit (Ambion by Life Technologies, USA, Cat. No.: 12183018A) as recommended by the manufacturer. One hundred and fifty nanograms of total RNA were labeled and then hybridized to the Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip (Agilent Technologies, Santa Clara, CA, USA). All microarray experiments were conducted at the Microarray Core Facility (Stem Cell Unit, King Saud University College of Medicine). Normalization and data analyses were conducted using the GeneSpring GX software (Agilent Technologies). Pathway analysis was conducted using the Single Experiment Pathway analysis feature in GeneSpring 12.0 (Agilent Technologies) as described in (ref. 5). Twofold cutoff with P<0.02 was used.
3
Gene Expression Profiling of Total RNA
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Total RNA was extracted using innuPREP RNA Mini Kit (Analytik Jena, Germany, REF No: 845-KS-2040250) as recommended by the manufacturer. 50 ng of total RNA was labeled and hybridized with the Agilent Human SurePrint G3 Human GE 8 × 60 k v16 microarray chip (Agilent Technologies). All microarray experiments were conducted at the Microarray Core Facility (Stem Cell Unit, Anatomy Department, College of Medicine, King Saud University). Normalization and data analyses were conducted using the GeneSpring GX software (Agilent Technologies). Pathway analysis was conducted using the Single Experiment Pathway analysis feature in GeneSpring 12.0 (Agilent Technologies). A two-fold cutoff with P < 0.05 was used.
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