Wrn 8h3 mouse mab

Manufactured by Cell Signaling Technology

Sourced in United States

WRN (8H3) mouse mAb is a monoclonal antibody that recognizes the Werner syndrome ATP-dependent helicase (WRN) protein. It is designed for use in western blotting, immunoprecipitation, and immunofluorescence applications.

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Lab products found in correlation

Mouse alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Anti mouse igg hrp, by Agilent Technologies (2 mentions) Rabbit anti phospho histone h2a x ser139, by Cell Signaling Technology (2 mentions) P0448, by Agilent Technologies (1 mentions) Mouse anti flag f1804, by Merck Group (1 mentions) Mouse anti gapdh ab8245, by Abcam (1 mentions) Rabbit anti actin, by Merck Group (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti gapdh, by Abcam (1 mentions)

2 protocols using wrn 8h3 mouse mab

Immunostaining Antibody Dilutions

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The antibodies used in this study are as follows: WRN (8H3) mouse mAb (4666, 1/1,000 dilution; Cell Signaling), mouse anti-FLAG (F1804; Sigma-Aldrich, 1/1,000 [immunoblotting] or 1/500 [immunofluorescence] dilution), rabbit anti–phospho-histone H2A.X (Ser139) (2577, 1/800 dilution; Cell Signaling), mouse anti-GAPDH (ab8245, 1/30,000 dilution; Abcam), mouse Alexa Fluor 488 (1/1,000 dilution; Molecular Probes) and secondary rabbit (P0448, 1/1,000 dilution; Dako), mouse anti-IgG-HRP (P0161, 1/1,000 dilution; Dako).

Newman J.A., Gavard A.E., Lieb S., Ravichandran M.C., Hauer K., Werni P., Geist L., Böttcher J., Engen J.R., Rumpel K., Samwer M., Petronczki M, & Gileadi O. (2020). Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancers. Life Science Alliance, 4(1), e202000795.

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Immunoblotting and Immunofluorescence Antibodies

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The following antibodies were used: WRN (8H3) mouse mAb (Cell Signaling, 4666, 1/1000 dilution), mouse anti-GAPDH (Abcam, ab8245, 1/30000 dilution), mouse anti-FLAG (SIGMA, F1804, 1/1000 [immunoblot] or 1/500 [immunofluorescence] dilution), mouse anti-LAP2ß (BD Transduction Laboratories #611000, 1/100 dilution), mouse anti-p53 (Calbiochem, OP43, 1/1000 dilution), rabbit anti-Actin (SIGMA, A2066, 1/4000) dilution), mouse anti-MLH1 (Cell Signaling, 3515, 1/1000 dilution), rabbit anti phospho-Histone H2A.X (Ser139) (Cell Signaling, 2577, 1/800 dilution) a rabbit anti-MSH3 (Santa Cruz, sc-11441, 1/500 dilution) and secondary rabbit (Dako, P0448, 1/1000 dilution), mouse anti-IgG-HRP (Dako, P0161, 1/1000 dilution) and mouse Alexa Fluor 488 (Molecular Probes, Eugene, OR, US, 1/1000 dilution).

Lieb S., Blaha-Ostermann S., Kamper E., Rippka J., Schwarz C., Ehrenhöfer-Wölfer K., Schlattl A., Wernitznig A., Lipp J.J., Nagasaka K., van der Lelij P., Bader G., Koi M., Goel A., Neumüller R.A., Peters J.M., Kraut N., Pearson M.A., Petronczki M, & Wöhrle S. (2019). Werner syndrome helicase is a selective vulnerability of microsatellite instability-high tumor cells. eLife, 8, e43333.

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