Lsm 7 mp 2 photon microscope

Manufactured by Zeiss

Sourced in Germany

The LSM 7 MP 2-photon microscope is a high-performance imaging system designed for advanced research applications. It utilizes two-photon excitation technology to enable deep tissue imaging with improved signal-to-noise ratio and reduced phototoxicity. The core function of this microscope is to provide researchers with a powerful tool for non-invasive, high-resolution imaging of living samples.

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Lab products found in correlation

Na water immersionobjective, by Zeiss (3 mentions) Dichroic mirror lp 760, by Zeiss (3 mentions)

Spelling variants of this product

LSM 7 MP (56 citations) LSM 7 MP microscope (13 citations) LSM 7 (12 citations) LSM 7 microscope (5 citations)

3 protocols using lsm 7 mp 2 photon microscope

Two-Photon Fluorescence Lifetime Imaging

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The fluorescence lifetime images were acquired using LSM 7 MP 2-
photon microscope (Carl Zeiss, Weimar, Germany) coupled to the Becker
and Hickl (BH) simple-Tau-152 system. Chameleon Ti:sapphire laser
system with an 80 MHz repetition rate was used to excite the sample
at a wavelength of 800 nm. Images were acquired through a Zeiss 20
× 1 NA water-immersion objective. A Zeiss dichroic mirror (LP
760) was used to separate the excitation and the emission light. Emission
light was collected using a hybrid GaAsP detector (HPM-100-40, BH,
Berlin, Germany). The image acquisition time was set to 150 s to collect
a sufficient number of photons.

Basavalingappa V., Bera S., Xue B., O’Donnell J., Guerin S., Cazade P.A., Yuan H., Haq E.U., Silien C., Tao K., Shimon L.J., Tofail S.A., Thompson D., Kolusheva S., Yang R., Cao Y, & Gazit E. (2020). Diphenylalanine-Derivative Peptide Assemblies with Increased Aromaticity Exhibit Metal-like Rigidity and High Piezoelectricity. ACS Nano, 14(6), 7025-7037.

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Fluorescence Lifetime Imaging of Photostability

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The fluorescence lifetime images were acquired using a LSM 7 MP 2-photon microscope (Carl Zeiss, Weimar, Germany) coupled to the Becker and Hickl (BH) simple-Tau-152 system. Chameleon Ti:sapphire laser system with an 80 MHz repetition rate was used to excite the sample at a wavelength of 1000 nm. Images were acquired through a Zeiss 20 × 1 NA water-immersion objective. A Zeiss dichroic mirror (LP 760) was used to separate the excitation and the emission light. Emission light between 625 and 760 nm was collected via a hybrid GaAsP detector (HPM-100-40, BH, Berlin, Germany). The image acquisition time was 150−180 s to collect a sufficient number of photons.
For the photostability measurements, 50 images were collected for 23 min every 25 s. Images were analyzed using the SPCImage software (BH).

Tao K., Xue B., Frere S., Slutsky I., Cao Y., Wang W, & Gazit E. (2017). Multiporous Supramolecular Microspheres for Artificial Photosynthesis. Chemistry of materials : a publication of the American Chemical Society, 29(10), 4454-4460.

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Fluorescence Lifetime Imaging Microscopy

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The fluorescence lifetime imaging was carried out using LSM 7 MP 2-photon microscope (Carl Zeiss, Weimar, Germany) attached to the Becker and Hickl (BH). The sample was excited at a wavelength of 1000 nm using Chameleon Ti:sapphire laser system with a repetition rate of 80 MHz. Fluorescent mages were collected through a Zeiss 20×1 NA water-immersion objective. Excitation and emission light were separated by a Zeiss dichroic mirror (LP 760) coupled to the instrument. Emission light was collected between wavelength of 300–500 nm through a hybrid GaAsP detector (HPM-100-40, BH, Berlin, Germany). The image acquisition time was set to 150–180 s to allow for sufficient number of photons to collect. Image analysis was done by SPCImage software (BH).

Basavalingappa V., Bera S., Xue B., Azuri I., Tang Y., Tao K., Shimon L.J., Sawaya M.R., Kolusheva S., Eisenberg D.S., Kronik L., Cao Y., Wei G, & Gazit E. (2019). Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid. Nature Communications, 10, 5256.

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