The UHPLC-MS/MS system consists of a Triple Quad™ LC-MS/MS 5500+ system (SCIEX, Framingham, MA, USA) and an Ultra Performance Liquid Chromatography UHPLC, ExionLC™ AD (Shimadzu, Kyoto, Japan). Xinzhi SB25-12DTD ultrasonic bath (Ningbo, China) was used for ultrasonic-assisted extraction. Vortex-Genie 2 (Scientific Industries, Bohemia, NY, USA) was used to mix the sample solution. A heating and drying oven (Jinghong DHG-9240A, Shanghai, China) was used for drying and heat treatment of samples. An electronic analytical balance (Mettler-Toledo ME204, Zurich, Switzerland) was used to weigh the samples accurately. A medical refrigerator (Aucma YCD-265, Qingdao, China) was used to store experimental samples. A 0.22 µm syringe filter (Biosharp life sciences, Hefei, China) was used for sample filtration. Centrifugation of the sample solution was performed using a 5418 high-speed centrifuge (Eppendorf Corp., Hamburg, Germany).
Exionlc ad
Manufactured by Shimadzu
Sourced in Japan
The ExionLC™ AD is a high-performance liquid chromatography system designed for analytical applications. It provides accurate and reliable separation and detection of a wide range of chemical compounds. The system features advanced technology to ensure consistent and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Me204, by Mettler Toledo (2 mentions)
Vortex genie 2, by Scientific Industries (2 mentions)
Api 5500, by AB Sciex (1 mentions)
0.22 μm syringe filter, by Biosharp (1 mentions)
Column oven, by Shimadzu (1 mentions)
Absciex 6500 qtrap mass spectrometer, by AB Sciex (1 mentions)
Acquity uplc hss t3 column, by Waters Corporation (1 mentions)
Analyst 1, by AB Sciex (1 mentions)
4 protocols using exionlc ad
1
UHPLC-MS/MS Analysis of Compounds
2
Quantitative Mass Spectrometry Analysis
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Mice or rats’ plasma, brain homogenate, and sodium phosphate buffer samples for all compounds were processed by protein precipitation with methanol-acetonitrile (1:1) and analyzed by an AB Sciex API 5500 plus tandem mass spectrometer equipped with a Shimadzu ExionLC AD binary high-pressure gradient pump controlled via PE-Sciex sample control software. Analyst 1.7.1 was used for data acquisition and quantitation.
3
UHPLC-MS/MS Analysis of Compounds
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The UHPLC-MS/MS system consisted of Triple Quad™ LC-MS/MS 5500+ (AB SCIEX, USA) and Ultra Performance Liquid Chromatography UHPLC, ExionLC™ AD (Shimadzu, Kyoto, Japan). A Xinzhi SB25-12DTD ultrasonic bath (Ningbo, China) was used for the supersonic-assisted extraction. A Vortex-Genie 2 (Scientific Industries, Bohemia, NY, USA) was employed to mix the sample solution. A heating and drying oven (Jinghong DHG-9240A, Shanghai, China) was used for drying and heating treatment of samples. An electronic analytical balance (Mettler-Toledo ME204, Zürich, Switzerland) was used to weigh samples precisely. A medical refrigerator (Aucma YCD-265, Qingdao, China) was used for storing experimental samples. A 0.22 μm syringe filter (Biosharp life sciences, Hefei, China) was used for sample filtration. Centrifugation of the sample solution was accomplished with a 5418 high-speed centrifuge (Eppendorf Corp., Hamburg, Germany).
4
LC-MS/MS Analysis of Chemical Compounds
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LC and MS conditions LC-MS/MS analysis was conducted on ExionLC AD consisting of binary pumps, an on-line degassing unit, an autosampler, and a column oven (Shimadzu Corporation, Kyoto, Japan), which is coupled with an AB Sciex 6500 + QTRAP mass spectrometer consisting of an electrospray ionization (ESI) source (AB SCIEX, Framingham, MA, USA). Chromatographic separation was achieved on Waters Acquity UPLC HSS T3 Column, 100Å, 1.8 μm, 2.1 mm X 100 mm maintained at 40 °C, at a flow rate of 0.3 mL/min. The mobile phases consisted of Solution A (0.1% Formic acid in water) and Solution B (100% acetonitrile). The following gradient was used: 0–1 min, 98% A; 1–5 min, 98%-45% A; 5–8 min, 45%-0% A; 8–13 min, 100% A; 13-13.1 min, 100%-2% A; 13.1–18 min, 98% A, with a total run time of 18 min. The ion source was operated in mix mode: curtain gas, 35 psi; nebulizer gas 50 psi; auxiliary gas 50 psi; ion spray voltage, 5500 V/-4500 V (positive/negative); and temperature 500 °C. Multiple reaction monitoring (MRM) transitions were identified for all analyses and isotope-labeled standard. Data acquisition and analysis were all performed with Analyst 1.6.3 software (AB SCIEX) and OS software (AB SCIEX).
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