Ammonium sulfate

The sodium phosphate buffer was prepared using di‐sodium hydrogen phosphate anhydrous (Na₂HPO₄, purity 99.0%) and sodium phosphate monobasic (NaH₂PO₄, purity 99–100.5%) both acquired at Panreac. Ammonium sulfate (purity 99%) and sodium azide (purity 99%) were purchased from Panreac and Acros Organics, respectively.

The three oils used in this study were coconut oil (Organic extra virgin coconut oil from PLANTIS^®, Barcelona, Spain), rapeseed oil (Organic cold pressed rapeseed oil Terpenic lab S.L., Barcelona, Spain) and grape seed oil (Refined grape seed Terpenic lab S.L., Barcelona, Spain). Three different batches of each type of oil were purchased in a local market.
2-thiobarbituric acid, and fatty acid methyl esters were obtained from Sigma-Aldrich Chemical (Steinheim, Germany). Boron/methanol trifluoride and heptane were purchased from Merck (Whitehouse Station, NJ, USA). Potassium hydroxide, hexane, cyclohexanone, hydrochloric acid, trichloroacetic acid and ammonium sulfate were from Panreac (Barcelona, Spain).
Salmonella typhimurium strains TA97a and TA98 and the Mutazime S9 mix at 10% from livers of Aroclor 1254-induced rats were purchased from Moltox (Boone, NC, USA). Nutrient broth and Phosphate Buffered Saline (PBS) tablets were obtained from Oxoid (Basingtone, UK). 4-nitro-o-phenylenediamine (NPD), 2-aminoanthracene (AA) and 2-aminofluorene (AF), histidine and biotin were obtained from Sigma-Aldrich.

Ultrapure reagent-grade water was obtained from a Milli-Q system (Millipore/Waters, Etten-Leur, The Netherlands). Ammonium sulfate, ethylenediamine tetra-acetic acid (EDTA) and sodium dodecyl sulfate (SDS) were obtained from PanReac Applichem (Darmstadt, Germany). Hydrochloric acid and Tween-20 were purchased from ThermoFisher Scientific (Waltham, MA, USA). Sodium chloride was obtained from Honeywell (Charlotte, NC, USA) Sodium deoxycholate was acquired from Sigma-Aldrich Co. (St. Louis, MO, USA). Tris-base was obtained from Fisher Scientific (Epson, UK). Nonidet P-40 (NP-40) was purchased from Fluka (Monte Carlo, Monaco). Bis-Acrylamide/Acrylamide 40% was bought from GRiSP Research Solutions (Oporto, Portugal). The NZYColour Protein Marker II was acquired from NZYTech (Lisbon, Portugal). All chemicals were of analytical-grade commercially available and used without further purification.