HPLC grade ammonium acetate, acetonitrile, methanol, chloroform, ethanol, hexane, and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standard compounds and isotope labeled standards, including N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Hplc grade ammonium acetate
Manufactured by Avantor
Sourced in Belgium
HPLC Grade Ammonium acetate is a high-purity salt used as a buffer in high-performance liquid chromatography (HPLC) mobile phases. It helps to maintain a stable pH and enhance the separation and resolution of analytes during HPLC analysis.
Automatically generated - may contain errors
Lab products found in correlation
Gibberellic acid, by Merck Group (2 mentions)
Jasmonic acid, by Merck Group (2 mentions)
Sarcosine d3, by Merck Group (2 mentions)
Thymine d4, by Merck Group (2 mentions)
Trans zeatin, by Merck Group (2 mentions)
Testosterone d3, by Merck Group (2 mentions)
Ms grade formic acid, by Merck Group (2 mentions)
N acetyl aspartic acid d3, by Merck Group (2 mentions)
Tryptophan 15n2, by Merck Group (2 mentions)
Glutamic acid d5, by Merck Group (2 mentions)
15n anthranilic acid, by Merck Group (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
High performance liquid chromatography (hplc), by Thermo Fisher Scientific (2 mentions)
Minocycline, by Merck Group (2 mentions)
Nhs peg mal 5000, by Merck Group (2 mentions)
Hplc grade glutaraldehyde, by Thermo Fisher Scientific (2 mentions)
Pd 10 columns sephadex g 25 m, by GE Healthcare (2 mentions)
Milli q plus apparatus, by Merck Group (2 mentions)
Hplc grade water, by Thermo Fisher Scientific (2 mentions)
5 protocols using hplc grade ammonium acetate
1
Metabolite Extraction and Quantification
2
Comprehensive Metabolite Profiling Approach
3
HPLC Buffer Preparation and Solvents
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Buffer solutions (polar solvents for HPLC experiments) were prepared by dissolving HPLC-grade ammonium acetate (VWR International, Leuven, Belgium) in ultrapure water obtained from a Milli-Q water purification system (Merck Millipore, Darmstadt, Germany). To adjust the buffer solutions’ pH, two concentrated solutions were used: ammonia (Avantor Performance Materials Poland S.A., Gliwice, Poland) and acetic acid (Chempur, Piekary Śląskie, Poland). HPLC-grade acetonitrile (Chempur, Piekary Śląskie, Poland) and isopropanol (VWR International, Leuven, Belgium) were used as non-polar solvents for HPLC experiments.
4
Minocycline-Albumin Nanoparticle Synthesis
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Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich Saint Louis, MO, USA. HPLC Grade Ammonium acetate was purchased from VWR. HPLC-grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) was obtained from Alfa Aesar (Ward Hill, MA, USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. All other chemicals used in this study were of analytical grade. Traut’s reagent (2-Iminothiolane) and Ellman’s reagent were obtained from Pierce (Rockford, IL, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Effendorf centrifuge 5810R, Thermo scientific sorvall RC 6+, Waltham, MA, USA), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
5
Minocycline-Based Drug Delivery Platform
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Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich U.S.A. HPLC Grade Ammonium acetate was purchased from VWR. HPLC grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) were obtained from Alfa Aesar (USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. Traut's reagent (2-Iminothiolane) and Ellman's reagent was obtained from Pierce (Rockford, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Eppendorf Centrifuge 5810R, Thermo scientific sorvall RC 6+), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
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