For protein expression studies, we used pRSET-A and pFN2A Flexi vectors (Thermo Scientific) with competent BL21(DE3) and DH5α cells (NEB, USA). pGAD-T7 Rec (Clontech) was used for the cDNA library preparation, pGADT7-AD (Clontech) as prey vector for one to one assays, and were then transformed in Y2H Gold yeast cells. pGBKT7 (Clontech) was used as a bait vector (DNA-BD) and was transformed into Y187 yeast cells.
Pfn2a flexi vectors
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PFN2A Flexi vectors are a set of plasmid vectors designed for the expression of recombinant proteins in various host systems. These vectors provide a flexible and versatile platform for the cloning and expression of genes of interest. The core function of the PFN2A Flexi vectors is to facilitate the efficient expression and purification of recombinant proteins.
Automatically generated - may contain errors
3 protocols using pfn2a flexi vectors
1
Protein Expression and Yeast Two-Hybrid
2
Protein Expression and Yeast Two-Hybrid
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For protein expression studies, we used pRSET-A and pFN2A Flexi vectors (Thermo Scientific) with competent BL21(DE3) and DH5α cells (NEB, USA). pGAD-T7 Rec (Clontech) was used for the cDNA library preparation, pGADT7-AD (Clontech) as prey vector for one to one assays, and were then transformed in Y2H Gold yeast cells. pGBKT7 (Clontech) was used as a bait vector (DNA-BD) and was transformed into Y187 yeast cells.
3
Protein Expression and Yeast Two-Hybrid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For protein expression studies, we used pRSET-A and pFN2A Flexi vectors (Thermo Scientific) with competent BL21 (DE3) and DH5α cells (NEB, USA). pGAD-T7 Rec (Clontech) was used for the cDNA library preparation, pGADT7-AD (Clontech) as prey vector for one to one assays and were transformed in Y2H Gold yeast cells. pGBKT7 (Clontech) was used as a bait vector (DNA-BD) and was transformed into Y187 yeast cells.
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