Paraffin sections of the urinary bladder were IHC stained by anti-Ki67 (1/25, monoclonal, MIB-5, Dako, Glostrup, Denmark) antibodies. Antibodies were applied on paraffin-embedded sections with 20-min heat-induced epitope retrieval in Dako Target Retrieval Solution, citrate pH 6, and 30-min incubation at room temperature with the primary antibody. Combination of biotinylated rabbit anti-mouse immunoglobulins (Dako, code No. E0464) diluted 1:50, and streptavidin/HRP, diluted 1:300, was used for visualization using DAB+ (DakoCytomation) as chromogen.
Mib 5
Manufactured by Agilent Technologies
Sourced in Denmark
The MIB-5 is a laboratory instrument designed for the analysis of trace-level contaminants in various sample matrices. It utilizes a multi-dimensional ion mobility spectrometry (MIB) technique to separate and detect a wide range of analytes. The core function of the MIB-5 is to provide highly sensitive and selective analytical capabilities for the identification and quantification of target compounds in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using mib 5
1
Immunohistochemical Ki67 Staining
2
Immunohistochemical Analysis of Thyroid Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The resected thyroid glands were fixed in 20% buffered formalin and then embedded in paraffin blocks to cut sections for immunohistochemistry. Deparaffinized 4-μm sections were pre-treated by autoclaving for 20 min at 120°C in target retrieval solution (pH 6.0) (Dako, Glostrup, Denmark). The sections were incubated for 1 h at 37°C with 1:50 dilution of anti-Ki-67 mouse monoclonal antibody (MIB-5, Dako) or 1:200 dilution of anti-p21 mouse monoclonal antibody (CP74, Abcam, Cambridge, UK) in a humidified chamber. After incubation with EnVision+ system-HRP-labeled polymer anti-mouse (Dako) for 60 min, immunoreactivity was visualized by incubation with 3,3-diaminobenzidine (DAB) using a liquid DAB+ substrate chromogen system (Dako). The number of Ki-67-positive cells in thyroid follicular cells was counted as the Ki-67 labeling index (LI; the number of positive cells per one high-power field). The level of p21 immunoreactivity in thyroid follicular cells was assessed by scoring the highest intensity of staining in one high-power field as follows: negative, 0; faint nuclear staining, 1; moderate nuclear staining, 2; intense nuclear and cytoplasmic staining, 3.
3
Quantifying Hepatocyte Proliferation via Ki-67 Immunostaining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In assessing hepatocyte proliferation, liver tissues from the hepatic lobes were immune stained with anti-Ki-67 antibodies (monoclonal mouse anti-human Ki-67 antigen; MIB-5; Dako, Carpinteria, California), which detects all active parts of the cell cycle and has a strong positive correlation with proliferating antigen expression, bromodeoxyuridine incorporation, and thymidine incorporation 12 (link). The immune stained sections were counterstained with hematoxylin. Ki-67-positive cells were counted in six medium-power fields (x200 magnification) per section. The proliferation index was defined as the percentage of Ki-67-positive hepatocytes per total hepatocyte count in the field of view. The criteria of nuclear labeling index for Ki67 in this study were as follows: four degrees of positive nuclei for Ki67 were identified: negative if <24%, weak positive (+) if 25-%50%, moderately positive (++) if 51%-74%, strongly positive (+++) if the total score was greater than or equal to 75%.
4
Immunohistochemical Analysis of Cerebral Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five μm histological sections of formalin-fixed, paraffin-embedded cerebrum tissue were placed on polylysine-coated glass slides. Tissue sections were deparaffinized in xylene after overnight packing at 65°C and rehydrated in alcohol descending grades. Endogenous peroxidase activity was removed by incubation of tissue sections in 3% H 2 O 2 for 10 minutes at room temperature. The tissue sections were placed in digested media composed of 0.05 % trypsin (pH 7.8) for 15 minutes at 37°C and incubated with the primary antibody of Glial fibrillary acidic protein GFAP, the primary antibody against proliferating cell nuclear antigen PCNA and amyloid-β (DAKO, clone MIB5, 1:50, mouse) at 1:50 overnight at 4°C. After washing, the slides were incubated at room temperature with secondary biotin linked anti-mouse antibody for 50 minutes, and with the streptavidin-peroxidase complex for 50 minutes, then washed and incubated with developing solution (diaminobenzidine-hydrogen peroxide; DAKO), and counterstained with hematoxylin. As a result of the immune reaction, brown cytoplasmic or nuclear labeling counterstained with hematoxylin was visualized. Incubated sections with 1% nonimmune serum phosphate buffer solution (PBS), the solution used as negative controls. The sections were examined by using a bright-field light Olympus microscope with a digital canon camera.
5
Immunohistochemical Detection of Ki-67
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin sections from specimens of the ovaries were stained immunohistochemically to detect Ki67 expression using a monoclonal anti-rat Ki-67 antibody at a dilution of 1/20 according to the guidelines supplied by the manufacturer (Clone MIB-5, Dako, Glostrup, Denmark).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!