Log In Sign up
The largest database of trusted experimental protocols

Donkey anti goat igg hrp

Manufactured by Agilent Technologies
Visit Supplier

Donkey anti-goat IgG HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and quantify goat immunoglobulin G (IgG) in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ecl plus detection kit, by Thermo Fisher Scientific (2 mentions) Donkey anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions) Clarity max ecl substrate, by Bio-Rad (1 mentions)

Close spelling variants of this product

Anti-TM

2 protocols using donkey anti goat igg hrp

1

Western Blot Analysis of HENMT1 in Testes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from 10 week-old adult testes (n = 3/genotype) using NP-40 buffer (Fluka). 20μg of protein was separated on a 12% SDS-PAGE gel, proteins transferred to PVDF membranes and then probed using HENMT1 and actin (Sigma Aldrich) antibodies. Bound antibody was detected using donkey anti-goat IgG HRP (Dako) secondary antibody and donkey anti-rabbit IgG HRP respectively and detected with enhanced chemiluminescence (ECL Plus) detection kit (Thermo Scientific).
Lim S.L., Qu Z.P., Kortschak R.D., Lawrence D.M., Geoghegan J., Hempfling A.L., Bergmann M., Goodnow C.C., Ormandy C.J., Wong L., Mann J., Scott H.S., Jamsai D., Adelson D.L, & O’Bryan M.K. (2015). HENMT1 and piRNA Stability Are Required for Adult Male Germ Cell Transposon Repression and to Define the Spermatogenic Program in the Mouse. PLoS Genetics, 11(10), e1005620.
+ Open protocol
+ Expand
2

Testis Protein Extraction and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from whole testis homogenates and isolated germ cell populations using RIPA buffer (50mM Tris-HCL; 1% NP-40; 0.1% SDS; 0.5% sodium deoxycholate; 0.9% NaCl; 5mM EDTA ph 7.4) plus protease inhibitor cocktail (Calbiochem). Extracted protein was separated on a 12% SDS-PAGE gel, transferred to PVDF membranes and probed using primary antibodies. Bound antibody was detected using donkey anti-goat IgG HRP and donkey anti-rabbit IgG HRP (Dako) secondary antibodies with enhanced chemiluminescence ECL Plus detection kit (Thermo Scientific) or Clarity Max ECL substrate (BioRad).
Dunleavy J.E., Okuda H., O’Connor A.E., Merriner D.J., O’Donnell L., Jamsai D., Bergmann M, & O’Bryan M.K. (2017). Katanin-like 2 (KATNAL2) functions in multiple aspects of haploid male germ cell development in the mouse. PLoS Genetics, 13(11), e1007078.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!