Ab216839

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab216839 is a purified antibody product. The core function of this product is to serve as a tool for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ab216839

Immunofluorescence Analysis of Myocardial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The treated myocardiocytes were cultured in 6-well plates for 12 h at 37°C, washed with PBS, fixed with 4% paraformaldehyde for 15 min at room temperature, and then incubated with 0.1% Triton X-100 and 1% BSA for 30 min at room temperature. Myocardiocytes were incubated with primary anti-mouse antibodies against Bim (1:1,000), CHOP (1:1,000), activating transcription factor 4 (ATF4; 1:1,000; product code ab216839; Abcam), inositol-requiring enzyme 1α (IRE1α; 1:1,000; product code ab37073; Abcam), thiobarbituric acid reactive substances (TBARS; 1:1,000; ab118970; Abcam), reactive oxygen species (ROS; 1:1,000; ab186027; Abcam), H₂O₂ (1:1,000; ab138874; Abcam) overnight at 4°C. Myocardiocytes were washed with PBS and incubated with corresponding anti-rabbit secondary antibody (1:2,000, product code ab7090; Abcam) for 2 h at 25°C. Subsequently, myocardiocytes were stained with 5% DAPI for 30 min at room temperature. Images of myocardiocytes were captured using a Zeiss confocal spectral microscope (Carl Zeiss AG) at a magnification of ×100.

Fang Y., Duan C., Chen S., Liu Z., Jiang B., Ai W., Wang L., Xie P, & Fang H. (2021). Tanshinone-IIA inhibits myocardial infarct via decreasing of the mitochondrial apoptotic signaling pathway in myocardiocytes. International Journal of Molecular Medicine, 48(2), 158.

+ Open protocol

+ Expand

Western Blotting of Kidney Fibrosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described11 using whole cell lysates and kidney tissue homogenates. The primary antibodies used were: fibronectin (FN) (Cat No. 66042-1-AP, Proteintech), collagen I (Col-I) (Cat No. 14695-1-AP, Proteintech), PERK (ab229912; Abcam, USA), ATF4 (ab216839, Abcam), CHOP (ab11419, Abcam), GSK-3β (ab93926, Abcam), p-GSK-3β-Ser9 (ab131097 Abcam), and p-GSK-3β-Tyr216 (Cat No. 29125-1-AP, Proteintech).

Wu Y., Bai Y., Feng Y., Zhang Q., Diao Z, & Liu W. (2023). Renalase Prevents Renal Fibrosis by Inhibiting Endoplasmic Reticulum Stress and Down-Regulating GSK-3β/Snail Signaling. International Journal of Medical Sciences, 20(5), 669-681.

+ Open protocol

+ Expand

Intestinal Crypt Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Equal amounts of total protein extracted from the intestinal crypt cells, were electrophoresed on 12% polyacrylamide/sodium dodecyl sulfate gel and transferred onto polyvinylidene fluoride membranes. After being blocked for 45 min, the membranes were incubated with primary antibodies for 1 h. Subsequently, membranes were incubated with horseradish peroxidase-coupled secondary antibodies for 1 h after 3 times washing with PBS. Finally, the immunoreactivity was detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA, WBKLS0500). The antibodies used were as follows: anti-HSP60 (Abcam, ab46798, 1:1000), anti-LONP1 (Cell Signal Technology, 28020, 1:1000), anti-CDKN1A/p21 (Abclonal, A2691, 1:100), anti-Phospho-eIF2α-S51 (Abclonal, AP0692, 1:1000), anti-ClpP (Abcam, ab124822, 1:1000), anti-ATF4 (Abcam, ab216839, 1:1000), anti-ATF5(Abcam, ab184923, 1:1000), anti-CHOP(Cell Signal Technology, 2895 S, 1:1000), anti-TOM20 (Proteintech, 11802-1-AP, 1:1000), anti-WNT4 (Bio-Techne, MAB4751, 1:1000), anti-SIRT7 (Abclonal, A22735, 1:1000), anti-Actin (HUABIO, ET1702-67, 1:3000), HRP Conjugated Goat anti-Mouse IgG (HUABIO, HA1006, 1:3000) and HRP Conjugated Goat anti-Rabbit IgG (HUABIO, HA1001,1:3000).

Yang L., Ruan Z., Lin X., Wang H., Xin Y., Tang H., Hu Z., Zhou Y., Wu Y., Wang J., Qin D., Lu G., Loomes K.M., Chan W.Y, & Liu X. (2024). NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations. Nature Communications, 15, 546.

+ Open protocol

+ Expand

Investigating Inflammatory Mechanisms in Atherosclerosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Synthetic human IMD_1-53 was from Phoenix Pharmaceuticals (Burlingame, CA, USA). Alzet Mini-osmotic Pumps (model 2006) were from DURECT Corp (Cupertino, CA, USA). Primary antibodies for IMD (sc-86272), β-actin (sc-47778), IL-1β (sc-7884), and all horseradish peroxidase (HRP)-conjugated secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibodies for CD68 (ab125212), α-actin (ab5694), glucose-regulated protein78 (GRP78, ab21865), activating transcription factor (ATF4, ab216839), cleaved ATF6 (ab203119), p-inositol-requiring kinase 1 alpha (p-IRE1α, ab48187), CHOP (ab10444), cleaved-caspase-3 (ab13847) were from Abcam PLC (Cambridge, UK). Apoptosis-associated speck-like protein containing CARD (ASC, SAB4501315) were from Sigma-Aldrich (St. Louis, MO, USA). Dylight-labeled secondary antibodies were from EarthOx Life Sciences (Millbrae, CA, USA). The kit for reverse transcription of RNA and SuperReal PreMix Plus for real-time PCR (TIANGEN Biotech, Beijing, China). The oil red O and taurine (Tau) were from Sigma-Aldrich (St. Louis, MO, USA). Oxidized human LDL (ox-LDL, YB-002) was from Yiyuan Biotechnology (Guangzhou, China). Other chemicals and reagents were of analytical grade.

Ren J.L., Chen Y., Zhang L.S., Zhang Y.R., Liu S.M., Yu Y.R., Jia M.Z., Tang C.S., Qi Y.F, & Lu W.W. (2021). Intermedin1-53 attenuates atherosclerotic plaque vulnerability by inhibiting CHOP-mediated apoptosis and inflammasome in macrophages. Cell Death & Disease, 12(5), 436.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!