Tumor necrosis factor (TNF)-α (300-01A) was purchased from PeproTech (Rocky Hill, NJ, USA). Resveratrol (SML0963) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Endothelial cell medium (ECM), fetal bovine serum (FBS), endothelial cell growth supplement (ECGS), and penicillin/streptomycin solution (P/S) (1001) were purchased from Sciencell (CA, USA). Fluorescein (FITC)-conjugated affiniPure Goat Anti-Rabbit IgG (H+L) (111-095-144) was purchased from Jackson ImmunoResearch Company (USA). Rabbit Anti-Factor VIII related antigen (BA0046) was purchased from Boster (China). SIRT1 (ab32441), SIRT2 (ab51023), SIRT3 (ab86671), SIRT4 (ab105039), SIRT5 (ab105040), SIRT6 (ab62739), SIRT7 (ab105038) antibodies, and BCA protein assay kit (ab102536) were provided by Abcam (Abcam, USA) [11 (link)].
Bca protein assay kit ab102536
Manufactured by Abcam
Sourced in United States
The BCA protein assay kit (ab102536) is a colorimetric assay used for the quantitative determination of total protein concentration in a sample. The kit utilizes the bicinchoninic acid (BCA) method to detect and quantify the total protein present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti factor 8 related antigen ba0046, by Boster Bio (2 mentions)
Ab7970, by Abcam (1 mentions)
Sb203580 s1076, by Selleck Chemicals (1 mentions)
Ab32441, by Abcam (1 mentions)
Tumor necrosis factor alpha tnf α 300 01a, by Thermo Fisher Scientific (1 mentions)
Ex527 s1541, by Selleck Chemicals (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Endothelial cell growth supplement (ecgs), by ScienCell (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Small interfering rna sirna, by GenePharma (1 mentions)
2 protocols using bca protein assay kit ab102536
1
Resveratrol Modulates SIRT Expression
2
Resveratrol Modulates Endothelial Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Resveratrol (SML0963) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Tumor necrosis factor-alpha (TNF-α) (300-01A) was obtained from PeproTech (Rocky Hill, NJ, USA). Endothelial cell medium, fetal bovine serum, endothelial cell growth supplement, and penicillin/streptomycin solution (1001) were purchased from Sciencell Research Laboratories (Carlsbad, CA, USA). SB203580 (S1076), EX527 (S1541), and pyrrolidine dithiocarbamate (PDTC) (S1809) were purchased from Selleck Chemicals (Houston, TX, USA). Fluorescein isothiocyanate (FITC)-conjugated affiniPure goat anti-rabbit IgG (H+L) (111-095-144) was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Rabbit anti-factor VIII-related antigen (BA0046) was purchased from Boster (Wuhan, China). Anti-CD40 (ab47021), SIRT1 (ab32441), p65 nuclear factor (NF)-κB (ab7970), phospho-p38 MAPK (ab4822) antibodies, BCA protein assay kit (ab102536) were obtained from Abcam (Abcam, Cambridge, MA, USA). Small interfering RNA (siRNA) specifically targeting SIRT1 and CD40 and a negative control siRNA (Nc-siRNA) were synthesized by GenePharma (Shanghai, China).
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