The miR-141-3p inhibitor, miR-141-3p mimics, and homologous negative control were obtained from GenePharma (Shanghai, China). RLE-6TN cells were transfected above oligonucleotides using Lipofectamine 2000 reagent (Invitrogen) at the final concentration of 50–100 mM following the manufacturer’s instructions. Lentiviral short hairpin RNA (shRNA) vector as well as shRNA negative control (sh-NC) vector were commercially serviced by GeneChem Inc. (Shanghai, China). The shRNA targeting ZEB1-AS1 was 5ʹ-CCGGGCGCTCCTGTTTATGTACTTACTCGAGTAAGTACAT AAACAGGAGCGCTTTTTTG-3ʹ. RLE-6TN cells were transfected with sh-NC and sh-ZEB1-AS1 [multiplicity of infection = 100] diluted by Enhanced Infection Solution (ENi.S, pH 7.4). RT-qPCR was used to validate the transfection efficiency.
Lentiviral short hairpin rna shrna vector
Manufactured by Genechem
Sourced in Puerto Rico
The Lentiviral short hairpin RNA (shRNA) vector is a tool used for gene knockdown experiments. It functions by delivering a short hairpin RNA sequence into target cells, which triggers the RNA interference (RNAi) pathway and results in the silencing or downregulation of the corresponding gene.
Automatically generated - may contain errors
2 protocols using lentiviral short hairpin rna shrna vector
1
Modulating miR-141-3p and ZEB1-AS1 in Lung Cells
2
Lentiviral Knockdown of MALAT1 in OS Cell Lines
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Lentiviral short hairpin RNA (shRNA) vector as well as shRNA control (shCtrl) vector were commercially serviced by GeneChem Inc. (Shanghai, P.R. China). OS cell lines SaoS-2 and MG-63 were transfected with shCtrl and sh-MALAT1 [multiplicity of infection (MOI) = 100] diluted by Enhanced Infection Solution (ENi.S, pH 7.4). Polybrene (10 μg/ml) was used to enhance the effect of infection. After 72 h of transfection, the density of green fluorescent protein was observed as transfected cells were observed with green fluorescence.
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