The largest database of trusted experimental protocols

2 protocols using nci h522

1

Cell Line Cultivation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell lines (including A549, HCC-827, NCI-H292, NCI-H226, NCI-H460, NCI-H522, HCT116 P53(-/-), HCT116 P53(+/+), and HEK293t) were purchased from the Chinese Academy of Science Committee on Type Culture Cell Bank (Shanghai, China) and cultured in RPMI 1640 (Gibco, USA) or DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA).
+ Open protocol
+ Expand
2

Lung Cancer Cell Lines Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols
In this experiment, we used lung cancer cell lines (HCC827, NCI–H358, NCI–H522, NCI– H1299, NCI– H460, and NCI–H226) and HeLa cell line. We purchased the HCC827, NCI–H358, NCI–H522, NCI– H1299, NCI– H460, and HeLa from the Korea Cell Line Bank, and we purchased the NCI–H226 from the American Type Culture Collection. Lung cancer cells (HCC827, NCI–H358, NCI–H522, NCI– H1299, NCI– H460, and NCI–H226) and HeLa cells were maintained in an RPMI 1640 medium (GIBCO BRL, Rockville, MD, USA) with 10% fetal bovine serum and antibiotics (100 units/mL penicillin and 100 mg/mL streptomycin).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!