β actin antibody 60008 1 ig

The synthetic routes of target compounds and their identification results are shown in the supporting information files. The compounds used in the biological assay were in DMSO with a stock concentration of 10 mM, and the maximum concentration of DMSO used was no more than 0.1%. All of the chemical reagents and biological reagents, including DMSO, were purchased from Sigma-Aldrich (St Louis, MO, USA). All cell lines used in study were purchased from Typical Culture Preservation Commission Cell Bank, Chinese Academy of Sciences (NCB). The antibodies used from Cell Signaling Technology (Beverly, MA, USA) were the PARP antibody (9542L), cleaved-PARP antibody ⁵⁶²⁵, anti-Caspase 3 antibody ⁹⁶⁶⁴, cleaved caspase-3 antibody ⁹⁶⁶⁴, Bcl-2 antibody ³⁴⁹⁸, Bax antibody ²⁷⁷², c-RAF antibody ⁹⁴²², Akt antibody (9272S), Hsp70 antibody ⁴⁸⁷⁶, Erk1/2 antibody ⁴³⁷⁰, and HER-2/ErbB2 antibody ²²⁴². The HIF-1a antibody (ab51608) was purchased from Abcam (Cambridge, MA, USA). The β-actin antibody (60008-1-Ig) was from the Proteintech Group (Wuhan Sanying Biotechnology, Hubei, China). Cisbio Bioassays in France supplied the HTRF transcreener ADP kit. Adriamycin (S1208) was purchased from Selleck (Texas, USA).