Pnp tr au

Manufactured by NanoWorld

Sourced in Switzerland

The PNP-Tr-Au is a lab equipment product designed for specialized applications. It consists of a porous nanoporous membrane coated with gold nanoparticles. The core function of this product is to serve as a platform for various experimental and analytical processes, though its specific intended uses are not included in this description.

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Lab products found in correlation

16 mercaptododecahexanoic acid, by Merck Group (1 mentions) 1 mercapto 1 undecanol, by Merck Group (1 mentions) Nanoscope 4 controller, by Veeco (1 mentions) Ntegra afm, by NT-MDT (1 mentions)

Close spelling variants of this product

PNP-TR-TL-Au PNP-TR

3 protocols using pnp tr au

Functionalization of Gold Cantilevers for Corneodesmosin Immobilization

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Gold cantilevers (PNP-Tr-Au,
Nanoworld) were thoroughly rinsed with water and ethanol, dried with
N₂ flow, and further cleaned in a UV-ozone chamber for
10 min. They were then immersed overnight in a 1 mM solution containing
a mixture of 16-mercaptododecahexanoic acid (10%; Sigma-Aldrich) and
1-mercapto-1-undecanol (90%; Sigma-Aldrich) in ethanol and protected
from light. For some experiments, 16-mercaptododecahexanoic acid was
used at 1 or 0.1%. Then, cantilevers were rinsed with ethanol, dried
with N₂, and immersed in an aqueous solution containing
10 mg mL^–1N-hydroxysuccinimide
(NHS) and 25 mg mL^–1 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide
(EDC) for 30 min. After rinsing with ultrapure water, the cantilevers
were immersed in a 0.1 mg mL^–1 corneodesmosin (CDSN,
LSBio) solution in PBS for 1 hour and finally rinsed with PBS.

Paiva T.O., Viljoen A., da Costa T.M., Geoghegan J.A, & Dufrêne Y.F. (2022). Interaction of the Staphylococcus aureus Surface Protein FnBPB with Corneodesmosin Involves Two Distinct, Extremely Strong Bonds. ACS Nanoscience Au, 3(1), 58-66.

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Atomic Force Microscopy for Hair Characterization

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An atomic force microscope (Multimode, Veeco, UK) with a Nanoscope IV controller was used for characterizing the hair samples in both ambient and liquid. Gold‐coated cantilevers with a nominal spring constant of 0.32 N m⁻¹ (PNP‐TR‐Au, Nanoworld, Switzerland) were used to acquire surface morphology and surface interactions. Force curves were collected over different regions with a 2 nN applied load.
During a force measurement, AFM cantilever is positioned above the hair sample that is fixed to a piece of indium, as described in a previous work [34 (link)]. It subsequently approaches the hair fibre in the normal direction, makes contact before retracting from the hair surface at a defined frequency. Surface interactions between the cantilever and the hair fibre are registered during this process via the movement of laser beam that is focus on the backside of the cantilever onto a photodetector. A detailed explanation can be found in a review article by Butt and colleague [27 ]. By treating the cantilever as a spring, it is possible to translate the electronic signal recorded by the photodetector to an actual force, with rigorously calibrated deflection sensitivity and spring constant of the cantilever. Results generated are usually presented as force recorded as a function of the z piezo displacement that is the relative distance between the cantilever tip and the hair fibre.

Labarre L., Squillace O., Liu Y., Fryer P.J., Kaur P., Whitaker S., Marsh J.M, & Zhang Z.J. (2023). Hair surface interactions against different chemical functional groups as a function of environment and hair condition. International Journal of Cosmetic Science, 45(2), 224-235.

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Quantifying Cell Membrane Mechanics

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All measurements were conducted using a commercial atomic force microscope (NTEGRA AFM, NT-MDT) and V-shaped silicon nitride AFM probes (PNP-TR-Au, Nanoworld) with a spring constant of 0.08 N/m and face angle of 35° at room temperature. The cantilever spring was calibrated by thermal noise fluctuation methods,⁴⁶ and the deflection sensitivity of each probe was calibrated by force-distance measurements on the bare glass area of the petri dish. For cell experiments, all force-distance curves were obtained from the central cytoplasmic region of the cell surface. At least 256 force-distance measurements were carried out per individual cell. The loading rates ranging from 235 – 6720 pN/s were calculated by multiplying the probe retracting rate by the effective spring constant of the cantilever-cell membrane system. A 50 μg/ml of free cRGD peptide solution was used for integrin blocking experiments. The scanning resolution was 256 × 256 pixels with a scan rate of 0.1 – 0.5 Hz, depending on the scanning areas of irregular cell size. The acquired images were flattened, if required, to eliminate the background noise and tilt from the surface using all unmasked portions of scan lines to calculate individual least-square fit polynomials for each line.

Alhalhooly L., Confeld M.I., Woo S.O., Mamnoon B., Jacobson R., Ghosh S., Kim J., Mallik S, & Choi Y. (2022). Single Molecule Force Probing of RGD-binding Integrins on Pancreatic Cancer Cells. ACS applied materials & interfaces, 14(6), 7671-7679.

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