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2 protocols using p cresyl sulfate d7

1

Metabolite Standards for Analytical Methods

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Sigma Aldrich (St Quentin Fallavier, France) provided indole-3-acetic acid, indoxyl sulfate, hippuric acid, kynurenic acid, TMAO, phenylalanine, kynurenine, indole-3-acetic acid-d5, tryptophan, tyrosine, sodium hydrogen phosphate, formic acid, and dimethylsulfoxide (DMSO). Phenylacetyl-L-glutamine, Phenylacetyl-L-glutamine-d5, PCS, kynurenine-d4, p-cresyl-sulfate-d7, p-cresyl glucuronide, hippuric acid-d5, p-cresyl glucuronide-d7, TMAO-d9, tyrosine-d4, tryptophan-d5, phenylalanine-d5, kynurenic acid-d5, and 3-carboxy-4-methyl-5-propyl-2-furan were provided by Toronto Research Chemicals (North York, Toronto, ON, Canada). VWR Chemicals (Radnor, PA, USA) supplied the sodium dihydrogen phosphate and sodium chloride. Fisher Scientific (Illkirch, France) provided the LC-MS-grade methanol, water, and acetonitrile.
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2

Liquid Chromatography-MS Analysis Protocol

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Liquid chromatography-MS-grade acetonitrile was purchased from JT Baker (Center Valley, PA, USA) and formic acid was obtained from Fluka (St. Louis, MO, USA). Amicon Ultra 0.5-mL centrifugal filters (MWCO 30 kDa) were purchased from Merck Millipore (Burlington, MA, USA). IS standard was purchased from Sigma–Aldrich (St. Louis, MO, USA) and pCS was purchased from AlsaChim (Illkirch-Graffenstaden, France). 3- Indoxyl sulfate-d4 potassium salt (IS-d4) was purchased from Toronto Research Chemicals (North York, ON, Canada) and p-cresyl sulfate-d7 (pCS-d7) was synthesized by Professor Ren-Shen Lee of Chang Gung University (Taoyuan City, Taiwan)
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