Gp91phox y

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

gp91phox−/y is a laboratory product used in research settings. It serves as a tool to study the function of the gp91phox gene, which is involved in the production of NADPH oxidase, an enzyme complex responsible for the generation of superoxide in certain cell types. The product provides a means to investigate the role of gp91phox in various biological processes.

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Lab products found in correlation

B6.129s cybbtm1din j, by Jackson ImmunoResearch (1 mentions) C57bl 6j wt, by Jackson ImmunoResearch (1 mentions) C57bl 6 wt, by Jackson ImmunoResearch (1 mentions)

2 protocols using gp91phox y

Investigating Oxidative Stress in CGD

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All studies were approved and conducted under the oversight of the Institutional Animal Care and Use Committee at the University of Texas Southwestern Medical Center. C57BL/6J (WT) and Nox2-deficient (B6.129S-Cybbtm1Din/J) mice (gp91^phox−/y) were obtained from Jackson Laboratory (Bar Harbor, ME, USA) and kept in a barrier facility with free access to standard rodent chow and water. CYBB encodes gp91^phox and is present on the X chromosome. CGD resulting from a mutation in CYBB is inherited in an X-linked recessive pattern; therefore, only male mice were used. Mice were age matched for all experiments.

Hook J.S., Cao M., Potera R.M., Alsmadi N.Z., Schmidtke D.W, & Moreland J.G. (2019). Nox2 Regulates Platelet Activation and NET Formation in the Lung. Frontiers in Immunology, 10, 1472.

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Viral and Bacterial Infection in Transgenic Mice

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Specific pathogen-free, C57BL/6 WT, gp91^phox-/y, gp91^phox−/−, p47^phox−/−, NOS2^−/− and Mafia mice were initially purchased from the Jackson Laboratory (Bar Harbor, ME) and bred at Albany Medical College following IACUC guidelines.
Viral challenge was performed with A/PR/8 or H1N1 CA4 administered i.n. to anesthetized, sex and age-matched adult mice in 50 μl of sterile PBS. Unless otherwise specified, bacterial co-infection was performed at 7 days after influenza infection. To induce bacterial pneumonia, anesthetized mice were inoculated i.n. with 50 μl of PBS containing 10⁷- 2×10⁸ CFU of community-acquired MRSA strain BAA-1695 (an isolate from patient sputum), or MRSA BAA-1692 (an isolate from human sinus) obtained from ATCC. Titers of virus stocks and viral levels in the bronchoalveolar lavage fluids (BALF) and lungs of infected mice were determined by plaque assays on MDCK cell monolayers (21 (link)). Bacterial burdens in the lungs and BALF were measured by sacrificing infected mice at the indicated time points, and plating serial 10-fold dilutions of each sample onto blood agar plates.

Sun K, & Metzger D.W. (2014). Influenza infection suppresses NADPH oxidase-dependent phagocytic bacterial clearance and enhances susceptibility to secondary MRSA infection. Journal of immunology (Baltimore, Md. : 1950), 192(7), 3301-3307.

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