CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs) (Promega, Madison, WI, USA) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest, Billerica, MA, USA) supplemented with 10% Fetal Bovine Serum (FBS, Biowest) and 200 mM L- Glutamine (Biowest) in the presence of G418 (250 µg/mL, InvivoGen, San Diego, CA, USA) and Hygromycin B Gold (50 µg/mL, InvivoGen) as selection antibiotics. The overexpression of hPD-L1 and TCR ligand in aAPCs and PD-1 in ECs were confirmed by flow cytometry and western blot analysis, respectively. PCR tests for Mycoplasma sp. contamination [50 (link)] were routinely performed and indicated negative results for both cell lines.
Hpd 1 ecs
Manufactured by Promega
Sourced in United States
The HPD-1 ECs is a lab equipment product designed for the detection and quantification of hydrogen peroxide (H2O2) in various biological samples. It functions as a sensitive and reliable tool for researchers studying oxidative stress and related cellular processes.
Automatically generated - may contain errors
2 protocols using hpd 1 ecs
1
Engineered Cell Lines for PD-1/PD-L1 Assays
2
Characterization of PD-L1 and PD-1 Expressing Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CHO
K-1 cells overexpressing hPD-L1 and
the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1
aAPCs) (Promega, Madison, WI) and Jurkat T cells overexpressing hPD-1
and carrying a luciferase reporter gene under the control of Nuclear
Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector
Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest,
Billerica, MA) supplemented with 10% Fetal Bovine Serum (FBS, Biowest)
and 200 mMl -glutamine (Biowest) in the presence of G418
(250 μg/mL, InvivoGen, San Diego, CA) and Hygromycin B Gold
(50 μg/mL, InvivoGen) as selection antibiotics. The overexpression
of hPD-L1 and TCR ligand in aAPCs and PD-1 in ECs was confirmed by
flow cytometry and Western blot analysis, respectively. PCR tests
for Mycoplasma sp. contamination33 (link) were routinely performed and indicated negative
results for both cell lines.
K-1 cells overexpressing hPD-L1 and
the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1
aAPCs) (Promega, Madison, WI) and Jurkat T cells overexpressing hPD-1
and carrying a luciferase reporter gene under the control of Nuclear
Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector
Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest,
Billerica, MA) supplemented with 10% Fetal Bovine Serum (FBS, Biowest)
and 200 mM
(250 μg/mL, InvivoGen, San Diego, CA) and Hygromycin B Gold
(50 μg/mL, InvivoGen) as selection antibiotics. The overexpression
of hPD-L1 and TCR ligand in aAPCs and PD-1 in ECs was confirmed by
flow cytometry and Western blot analysis, respectively. PCR tests
for Mycoplasma sp. contamination33 (link) were routinely performed and indicated negative
results for both cell lines.
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