RPE cells were left untreated or treated with 1 μM A-1155463, 1 μM 4NQO, or both compounds for 2 h. The mitochondrial potential was determined using JC-1 dye staining according to the manufacturer’s instructions (ThermoFisher Scientific). The cells were washed once with PBS and stained with 10 ug/mL of JC-1 dye in culture medium for 10 min. The images of JC-1 staining were captured using EVOS fluorescence microscope and the mitochondrial potential was indicated by the shift of the fluorescence intensity ratio of J-aggregate (535/590 nm) and monomers (485/530 nm).
Jc 1 dye staining
Manufactured by Thermo Fisher Scientific
JC-1 dye is a fluorescent probe used to detect changes in mitochondrial membrane potential. It exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (525 nm) to red (590 nm).
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2 protocols using jc 1 dye staining
1
Mitochondrial Potential Assessment in RPE Cells
2
Mitochondrial Potential Assay in RPE Cells
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RPE cells were remained untreated or treated with 1 μM A-1155463, 1 μM 4NQO or their combination for 2 h. The mitochondrial potential was determined using JC-1 dye staining according to the manufacturer's instruction (ThermoFisher Scientific). The cells were washed once with PBS and stained with 10 ug/ml of JC-1 dye in culture medium for 10 min. The images of JC-1 staining were captured using EVOS fluorescence microscope and the mitochondrial potential was indicated by the shift of the fluorescence intensity ratio of J-aggregate (535/590 nm) and monomers (485/530 nm).
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