Escherichia coli BL21-CodonPlus-RIL cells (Stratagene, La Jolla, CA) harboring the plasmid pREP 4 and pQE-30 (both Qiagen, Venlo, Netherland) encoding either rPA83 or rBclA coupled to a HIS-tag were grown and purified as described previously [34 (link)]. Briefly, bacterial inoccula were grown in antibiotic supplemented Standard medium I (Roth, Karlsruhe, Germany). Upon reaching OD600nm of 0.6, protein production was induced with IPTG (Roth, Karlsruhe, Germany). After incubation for 3 h at 37 °C shaking cultures were lysed and proteins purified on a HiTrap Sepharose column loaded with nickel(II)sulfate (Sigma-Aldrich, Taufkirchen, Germany) via FPLC with Äktaprime plus (GE Healthcare, Freiburg, Germany). After dialysis onto 5 mM HEPES-buffer (Roth, Karlsruhe, Germany) and quality assessment via SDS-page, protein yield was established via Bicinchoninsäure test (Interchim, Montlucon, France). Proteins used for ELISA received no further treatment while proteins used for vaccination were tested for endotoxin (ET) yield using the Limulus Amoebocyte Lysate Endochrome-K test kit (Charles River, Wilmington, MA) according to the manufactures’s instructions. Endotoxin removal was performed via EndoTrap blue endotoxin removal system (Hyglos, Bernried, Germany) for proteins with ET values more than 50 ng/kg body weight.
Nickel 2 sulfate
Manufactured by Merck Group
Sourced in Germany
Nickel(II) sulfate is a chemical compound with the formula NiSO4. It is a green crystalline solid that is soluble in water. Nickel(II) sulfate is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Titanium tetrachloride, by Merck Group (2 mentions)
Nickel 2 chloride, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Phosphoric acid, by Merck Group (2 mentions)
Sodium sulfate, by Merck Group (2 mentions)
Boric acid, by Merck Group (2 mentions)
Pqe30, by Qiagen (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Carl Roth (1 mentions)
Ktaprime plus, by GE Healthcare (1 mentions)
Prep4, by Qiagen (1 mentions)
Hepes buffer, by Carl Roth (1 mentions)
Limulus amoebocyte lysate endochrome k test kit, by Charles River Laboratories (1 mentions)
Copper 2 nitrate trihydrate, by Merck Group (1 mentions)
Copper 2 chloride, by Merck Group (1 mentions)
Mercuric chloride, by Merck Group (1 mentions)
Ferric chloride, by HiMedia (1 mentions)
Tin chloride, by Merck Group (1 mentions)
Manganese chloride, by HiMedia (1 mentions)
Copper sulfate, by HiMedia (1 mentions)
6 protocols using nickel 2 sulfate
1
Purification of Recombinant Anthrax Proteins
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Escherichia coli BL21-CodonPlus-RIL cells (Stratagene, La Jolla, CA) harboring the plasmid pREP 4 and pQE-30 (both Qiagen, Venlo, Netherland) encoding either rPA83 or rBclA coupled to a HIS-tag were grown and purified as described previously [34 (link)]. Briefly, bacterial inoccula were grown in antibiotic supplemented Standard medium I (Roth, Karlsruhe, Germany). Upon reaching OD600nm of 0.6, protein production was induced with IPTG (Roth, Karlsruhe, Germany). After incubation for 3 h at 37 °C shaking cultures were lysed and proteins purified on a HiTrap Sepharose column loaded with nickel(II)sulfate (Sigma-Aldrich, Taufkirchen, Germany) via FPLC with Äktaprime plus (GE Healthcare, Freiburg, Germany). After dialysis onto 5 mM HEPES-buffer (Roth, Karlsruhe, Germany) and quality assessment via SDS-page, protein yield was established via Bicinchoninsäure test (Interchim, Montlucon, France). Proteins used for ELISA received no further treatment while proteins used for vaccination were tested for endotoxin (ET) yield using the Limulus Amoebocyte Lysate Endochrome-K test kit (Charles River, Wilmington, MA) according to the manufactures’s instructions. Endotoxin removal was performed via EndoTrap blue endotoxin removal system (Hyglos, Bernried, Germany) for proteins with ET values more than 50 ng/kg body weight.
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Escherichia coli BL21-CodonPlus-RIL cells (Stratagene, La Jolla, CA) harboring the plasmid pREP 4 and pQE-30 (both Qiagen, Venlo, Netherland) encoding either rPA83 or rBclA coupled to a HIS-tag were grown and purified as described previously [34 (link)]. Briefly, bacterial inoccula were grown in antibiotic supplemented Standard medium I (Roth, Karlsruhe, Germany). Upon reaching OD600nm of 0.6, protein production was induced with IPTG (Roth, Karlsruhe, Germany). After incubation for 3 h at 37 °C shaking cultures were lysed and proteins purified on a HiTrap Sepharose column loaded with nickel(II)sulfate (Sigma-Aldrich, Taufkirchen, Germany) via FPLC with Äktaprime plus (GE Healthcare, Freiburg, Germany). After dialysis onto 5 mM HEPES-buffer (Roth, Karlsruhe, Germany) and quality assessment via SDS-page, protein yield was established via Bicinchoninsäure test (Interchim, Montlucon, France). Proteins used for ELISA received no further treatment while proteins used for vaccination were tested for endotoxin (ET) yield using the Limulus Amoebocyte Lysate Endochrome-K test kit (Charles River, Wilmington, MA) according to the manufactures’s instructions. Endotoxin removal was performed via EndoTrap blue endotoxin removal system (Hyglos, Bernried, Germany) for proteins with ET values more than 50 ng/kg body weight.
2
Synthesis of Photocatalytic Hybrid Materials
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Copper(II) nitrate trihydrate (Cu(NO3)2.nH2O) (98%), peroxotungstic acid (H2W2O11) (95%), nickel(II) sulfate (NiSO4) (98%), nickel(II) chloride (NiCl2) (98%), boric acid (H3BO3) (97%), sodium hydroxide (NaOH) (99%), titanium tetrachloride (TiCl4) (99%), phosphoric acid (H3PO4) (70%), sodium sulfate (Na2SO4) (≥99%), copper(II) chloride (CuCl2) (98%), and acetone (C3H6O) (99.8%) were utilized as obtained from Sigma Aldrich. The Loctite 3430 insulating epoxy was used. Thick Al foil (99.99%) and Copper Cu tape (AT528) was acquired from Advance Tapes., while the TEC-15 fluorine-doped tin oxide (FTO) glass (density: 150 nm/1.1 mm; resistivity of sheet: ≤20 Ω/cm2, transmissivity: 88.9% wavelength 400 nm) was bought from NGS Glass.
3
Synthesis of Metal-Protein Conjugates
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Auric chloride (AuCl3), EST from
porcine liver, and rhodamine 6G were procured from Sigma-Aldrich (USA).
Various metal ion salts were purchased from different vendors, that
is, mercuric chloride, nickel(II) sulfate, zinc(II) acetate, and tin
chloride from Sigma-Aldrich, USA; calcium chloride, cobalt chloride,
sodium selenite, copper sulfate, aluminium sulfate, ferric chloride,
and manganese chloride from Himedia, India; cadmium chloride from
Qualigenes, India; magnesium chloride from, CDH, New Delhi, India;
and lead(II) acetate from Merck, India. Deionized water (Viola), NaOH
(Avra, India) and HCl (RANKEM, India) were used in the experiments.
Various other analytical grade chemicals and organic solvents were
purchased from the suitable vendors.
porcine liver, and rhodamine 6G were procured from Sigma-Aldrich (USA).
Various metal ion salts were purchased from different vendors, that
is, mercuric chloride, nickel(II) sulfate, zinc(II) acetate, and tin
chloride from Sigma-Aldrich, USA; calcium chloride, cobalt chloride,
sodium selenite, copper sulfate, aluminium sulfate, ferric chloride,
and manganese chloride from Himedia, India; cadmium chloride from
Qualigenes, India; magnesium chloride from, CDH, New Delhi, India;
and lead(II) acetate from Merck, India. Deionized water (Viola), NaOH
(Avra, India) and HCl (RANKEM, India) were used in the experiments.
Various other analytical grade chemicals and organic solvents were
purchased from the suitable vendors.
4
Electrochemical Metal Ion Sensors
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Polyvinyl
chloride (PVC), bis ethylhexyl
sebacate (DOS), bathophenanthroline (BP), 8-hydroxyquinoline (8HQ),
bathocuproine (BC), tetrahydrofuran (THF), dichloromethane (DCM),
sodium tetrakis-[3,5-bis(trifluoromethyl)phenyl]borate (NaBARF; Selectophore),
sodium acetate, glacial acetic acid, Dulbecco’s phosphate-buffered
saline (PBS), ammonium iron(II) sulfate hexahydrate, iron(III) chloride,
cobalt(II) sulfate hydrate, copper(II) sulfate, nickel(II) sulfate,
magnesium sulfate, and thioglycolic acid were all purchased from Sigma-Aldrich
(St. Louis, MI, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate
(DiO; Invitrogen) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,
4-chlorobenzenesulfonate salt (DiD; Invitrogen) were purchased from
Thermo Fisher Scientific (Waltham, MA, USA). Microdialysis hollow
fibers (13 kDa cutoff) were purchased from Spectrum Laboratories,
Repligen (Waltham, MA, USA). Ferrous sulfate supplement tablets were
purchased from Signature Care. Creek water was obtained from Clear
Creek in Golden, CO, USA on February 21, 2021.
chloride (PVC), bis ethylhexyl
sebacate (DOS), bathophenanthroline (BP), 8-hydroxyquinoline (8HQ),
bathocuproine (BC), tetrahydrofuran (THF), dichloromethane (DCM),
sodium tetrakis-[3,5-bis(trifluoromethyl)phenyl]borate (NaBARF; Selectophore),
sodium acetate, glacial acetic acid, Dulbecco’s phosphate-buffered
saline (PBS), ammonium iron(II) sulfate hexahydrate, iron(III) chloride,
cobalt(II) sulfate hydrate, copper(II) sulfate, nickel(II) sulfate,
magnesium sulfate, and thioglycolic acid were all purchased from Sigma-Aldrich
(St. Louis, MI, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate
(DiO; Invitrogen) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,
4-chlorobenzenesulfonate salt (DiD; Invitrogen) were purchased from
Thermo Fisher Scientific (Waltham, MA, USA). Microdialysis hollow
fibers (13 kDa cutoff) were purchased from Spectrum Laboratories,
Repligen (Waltham, MA, USA). Ferrous sulfate supplement tablets were
purchased from Signature Care. Creek water was obtained from Clear
Creek in Golden, CO, USA on February 21, 2021.
5
Laccase Purification and Characterization
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Copper(II) sulphate, hydrogen tetrachloroaurate(III) trihydrate, cerium(III) chloride, nickel(II) sulfate, chloroplatinic(IV) acid, palladium(III) chloride, o-dianisidine, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS), catechol, and other reagents and solvents used in this work were purchased from Sigma-Aldrich (Steinheim, Germany). All reagents were of analytical grade and were used without additional purification. All solutions were prepared using ultrapure water. Laccase from Trametes zonatus was purified and characterized as described by us earlier [18 (link),19 (link)].
6
Fabrication of Dye-Sensitized Solar Cells
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Si wafer (99%), nickel(II) sulfate (NiSO4; 98%), nickel(II) chloride (NiCl2; 98%), boric acid (H3BO3; 97%), sodium hydroxide (NaOH; 99%), titanium tetrachloride (TiCl4; 99%), phosphoric acid (H3PO4; 70%), sodium sulfate (Na2SO4; ≥99%), copper(II) chloride (CuCl2; 98%), acetone (C3H6O; 99.8%), polymethyl methacrylate (PMMA; (C5O2H8)n), and granular ZnO (99%) were utilized as obtained from Sigma Aldrich (Singapore, Singapore). The Loctite 3430 insulating epoxy was used. Thick Al foil (99.99%) and Copper Cu tape (AT528) were acquired from Advance Tapes (Surabaya, Indonesia), while the TEC-15 FTO glass (density 150 nm/1.1 mm; resistivity of sheet ≤ 20 W/cm2, transmissivity 88.9%; wavelength 400 nm) was bought from NSG Glass(Tokyo, Japan).
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