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Jes5 2a5

Manufactured by BioXCell
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Sourced in United States

The JES5-2A5 is a compact and versatile laboratory centrifuge. It is designed to provide efficient separation of substances within a sample. The centrifuge features a maximum rotor speed of 5,000 RPM and can accommodate a variety of sample tube sizes. The device is suitable for a range of applications in life science and clinical laboratories.

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Lab products found in correlation

Anti rat igg1, by BioXCell (2 mentions) Np klh, by Biosearch Technologies (1 mentions) Np lps, by Biosearch Technologies (1 mentions) Np klh, by Merck Group (1 mentions) Anti tgf β antibody 1d11, by BioXCell (1 mentions) Complete freund s adjuvant, by Merck Group (1 mentions) Imject alum, by Thermo Fisher Scientific (1 mentions) Pk136, by BioXCell (1 mentions) Sch58261, by Merck Group (1 mentions) Rmil 4, by BioLegend (1 mentions) Rmgm csf, by Merck Group (1 mentions) Mc 21, by BioXCell (1 mentions) Rmil 33, by BioLegend (1 mentions) Trfk5, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Anti ly6g 1a8, by BioXCell (1 mentions) Anti gr 1 rb6.8c5, by BioXCell (1 mentions) Anti cd25 pc 61.5.3, by BioXCell (1 mentions)

6 protocols using jes5 2a5

1

Modulation of Humoral Immune Responses

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Sex- and age-matched C57BL/6J mice were intraperitoneally (i.p.) administered 200 µg NP-KLH (Biosearch Technologies, Novato, CA, USA) emulsified with an equal amount of Imject Alum (Thermo Scientific), 100 µg NP-KLH emulsified with complete Freund’s adjuvants (Sigma), or 20–100 µg NP-LPS (Biosearch Technologies). Mouse sera were collected for ELISA, and isolated splenocytes were analyzed by flow cytometry at day 7 following the immunization. pCAGGS-Il10, pCAGGS-Tgfb1, and/or pCAGGS-Tgfb3 vectors were administered i.v. 1 day before the indicated immunization. Anti-IL-10 mAb (300 µg) (JES5-2A5, Bio X Cell, West Lebanon, NH, USA) and 300 µg anti-TGF-β antibody (1D11.16.8, Bio X Cell) were administered i.p. at day −1, 2, and 5.
Komai T., Inoue M., Okamura T., Morita K., Iwasaki Y., Sumitomo S., Shoda H., Yamamoto K, & Fujio K. (2018). Transforming Growth Factor-β and Interleukin-10 Synergistically Regulate Humoral Immunity via Modulating Metabolic Signals. Frontiers in Immunology, 9, 1364.
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2

Murine Allergic Airway Inflammation Model

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Mice were anesthetized by isofluorane inhalation, followed by the intranasal administration of rmIL-33 (0.2 µg, Biolegend), rmIL-4 (0.5 µg), rmIL-13 (0.5 µg), rmGM-CSF (0.5 µg), rmIL-5 (0.5 µg), LPS (1 μg, Sigma), CpG (10 μg, Invivogen), Aspergillus protease allergen (0.01U, Sigma), Ragweed pollen extract (300 μg, Greer) or clodronate liposome (C.L.) (30% C.L./PBS, Liposoma B.V.) in 40 µl of PBS. Diphtheria toxin (10 ng/g, Sigma), IL-33R-Fc (10 mg/kg, AstraZeneca), anti-NK1.1 mAb (50 μg, PK136, BioXcell), anti-CCR2 (20 μg, MC-21, provided by Prof. Matthias Mack52 (link)), anti-CCL2 (200 μg, MCP-1, BioXcell), anti-Ly6C/G (200 μg, GR-1, BioXcell), anti-Ly-6G (200 μg, 1A8, BioXcell), anti-CD4 (100 μg, GK1.5, BioXcell), anti-IL-10 (300 μg, JES5-2A5, BioXcell), anti-TGF-β (400 μg, 1D11.16.8, BioXcell), anti-IL-5 (100 μg, TRFK5, BioXcell), rat IgG1, κ (BioXcell), rat IgG2a (BioXcell), or rmIL-33 (0.5 µg, Biolegend) was administered by intraperitoneal injection in 100 µl of PBS. A2AR antagonist (20 μg, SCH 58261, Sigma) was administered in DMSO/PBS (v/v).
Schuijs M.J., Png S., Richard A.C., Tsyben A., Hamm G., Stockis J., Garcia C., Pinaud S., Nicholls A., Ros X.R., Su J., Eldridge M.D., Riedel A., Serrao E.M., Rodewald H.R., Mack M., Shields J.D., Cohen E.S., McKenzie A.N., Goodwin R.J., Brindle K.M., Marioni J.C, & Halim T.Y. (2020). ILC2-driven innate immune checkpoint mechanism antagonizes NK cell anti-metastatic function in the lung. Nature immunology, 21(9), 998-1009.
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3

Neutralizing IL-10 Enhances Antitumor CD8+ T Cells

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Neutralization of IL-10 was performed using an anti-IL-10 antibody (JES5-2A5, BioXCell) or isotype control IgG antibody (HRPN, BioXCell). Mice were intraperitoneally injected with 200 μg antibody in 12 h and 3 h before intravital imaging, respectively. Intravital imaging was performed 1 day after transferring activated OT-I CD8+ T cells into mice (4 days after tumor inoculation).
Liu L., Dai B., Li R., Liu Z, & Zhang Z. (2021). Intravital molecular imaging reveals the restrained capacity of CTLs in the killing of tumor cells in the liver. Theranostics, 11(1), 194-208.
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4

Neutralizing IL-10 in Mice

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Neutralization of IL-10 was performed using anti-IL-10 antibody (JES5-2A5, BioXCell) or isotype control anti-Rat IgG1 (BioXCell). Mice received 250 μg of antibody intra-peritoneally on days -1, 1, 3, 6, 9, and 12 relative to cell implantation.
de Almeida P.E., Meyer E.H., Kooreman N.G., Diecke S., Dey D., Sanchez-Freire V., Hu S., Ebert A., Odegaard J., Mordwinkin N., Brouwer T.P., Lo D., Montoro D., Longaker M.T., Negrin R.S, & Wu J.C. (2014). Transplanted Terminally Differentiated Induced Pluripotent Stem Cells Are Accepted By Immune Mechanisms Similar To Self-Tolerance. Nature communications, 5, 3903.
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5

Neutralizing IL-10 in Mice

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Neutralization of IL-10 was performed using anti-IL-10 antibody (JES5-2A5, BioXCell) or isotype control anti-Rat IgG1 (BioXCell). Mice received 250 μg of antibody intra-peritoneally on days -1, 1, 3, 6, 9, and 12 relative to cell implantation.
de Almeida P.E., Meyer E.H., Kooreman N.G., Diecke S., Dey D., Sanchez-Freire V., Hu S., Ebert A., Odegaard J., Mordwinkin N., Brouwer T.P., Lo D., Montoro D., Longaker M.T., Negrin R.S, & Wu J.C. (2014). Transplanted Terminally Differentiated Induced Pluripotent Stem Cells Are Accepted By Immune Mechanisms Similar To Self-Tolerance. Nature communications, 5, 3903.
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6

Immunomodulation Techniques in Mice

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For adjuvant/alum administration, mice received either saline or alum 200 mL intraperitoneal (IP) (Sigma-Aldrich) depending on the experimental schematic described in each figure. For IL-10 blockade, anti-IL-10 antibody (JES5-2A5, Bio X cell) was injected IP at a dose of 200 μg/mouse at time points described below. For MDSC depletion, 250μg anti-Ly6G (1A8, Bio X Cell) or anti-Gr1 (RB6-8C5, Bio X Cell) were injected IP 13 . For Treg depletion, 200μg/mouse of anti-CD25 (PC-61.5.3, Bio X cell) was injected IP as indicated as previously described 14 (link) .
Ge J., Pan W., Feeney N.J., Ott L., Anderson E., Alessandrini A., Zanoni I., Markmann J.F, & Cuenca A.G. (2023). Adjuvant conditioning induces an immunosuppressive milieu that delays alloislet rejection through the expansion of myeloid-derived suppressor cells. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 23(7).
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