A Coulter Counter Z2 (Beckman Coulter) was used with a 100-μm aperture and Z2 AccuComp v3.01a software. Except where indicated, samples were diluted ≥20-fold into Isoton II electrolyte solution in Acuvette cuvettes (Beckman Coulter) before counting, and the instrument was set to detect particles of 6–14 μm with a 500-μl metering volume. (The impedance pulses detected by the instrument are proportional to particle volumes. However, in the Coulter Counter documentation and literature, particle size is customarily indicated as a linear dimension, namely the diameter of a presumed perfect sphere of that volume. We also follow that practice here.) To create a particle-size-distribution profile, data from the Z2 were imported into Prism6.0a (GraphPad Software).
Accucomp
Manufactured by Beckman Coulter
The AccuComp is a laboratory instrument designed for the precise and reliable measurement of sample volumes. It utilizes advanced technology to ensure accurate liquid handling, a core function essential for various analytical applications in research and clinical settings.
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Lab products found in correlation
2 protocols using accucomp
1
Cell Size Analysis Using Coulter Counter
2
Cell Diameter Measurement using Coulter Counter
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Mean cell diameter (d = 2 x r) was measured using a Z2TM Coulter Counter ® (Beckman Coulter, Mississauga, ON) with an aperture diameter of 100 m. Analysis of the size distributions was then performed with the associated Accucomp ® software package (Beckman Coulter). In the case of HEK293 cell cultures, cellular aggregates could affect the measurement. Consequently, cell aggregates were described by Côté et al. (1998) . This step was not necessary in the case of Sf9 cells.
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