The immuno-phenotype (HLA-ABC, HLA-DR, CD40 and CD80) of hUCMSCs and day 21 IPCs was examined by flow cytometry on a flow cytometer (Epics Altra; Beckman Coulter) with specific phycoerythrin or FITC-conjugated monoclonal antibodies (eBioscience, Inc.) and performed as previously described (20 (link)). CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA) was used for data analysis. Results are expressed as a percentage of positive cells or as mean relative fluorescence intensity, obtained as a ratio between the mean fluorescence intensity of cells stained with specific mAb and the mean fluorescence intensity obtained with isotype control.
Fitc conjugated monoclonal antibodies
Manufactured by Thermo Fisher Scientific
FITC-conjugated monoclonal antibodies are fluorescently labeled immunoglobulin molecules that can be used to detect and visualize specific target proteins or cells in biological samples. The fluorescein isothiocyanate (FITC) dye is covalently attached to the antibody, allowing for the detection of the labeled antibody using fluorescence-based techniques.
Automatically generated - may contain errors
2 protocols using fitc conjugated monoclonal antibodies
1
Immunophenotyping of hUCMSCs and IPCs
2
Immunophenotyping of Mesenchymal Progenitor Cells
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The cell surface antigen profile of paired mCPCs (n = 6 donors) was analyzed by flow cytometry. mCPCs at passage 3 were harvested by trypsin digestion, and antibodies were stained individually (phycoerythrin (PE)-conjugated monoclonal antibodies against human CD29, CD44, CD73, and CD166; fluorescein isothiocyanate (FITC)-conjugated monoclonal antibodies against human CD45, CD90, and CD271; and allophycocyanin (APC)-conjugated antibodies against CD31 and CD105; eBioscience) for 30 min in the dark at 4 °C. After 2 washes with phosphate-buffered saline (PBS), events were collected by flow cytometry with a FACScalibur system (BectoneDickinson), and the data were analyzed using FlowJo 7.6 software.
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