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2 aminoethyl diphenylborinate a16606 2 apb

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2-Aminoethyl diphenylborinate (A16606) (2-APB) is a chemical compound used in research applications. It functions as a cell-permeable modulator of calcium signaling pathways.

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2 protocols using 2 aminoethyl diphenylborinate a16606 2 apb

1

Breast Cancer Cell Line Characterization

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The MDA-MB-231 human breast cancer (HTB-26) cell line was purchased from ATCC (Manassas, VA, USA). The passage numbers of cells used in experiments ranged from 5 to 20. thapsigargin (10522) (TG), fura-2-acetoxymethyl ester (14591) (Fura-2), N,N′-[1,2-ethanediylbis[(oxy-2,1-phenylene)]]bis[N-[2-(acetyloxy)methoxyl]-2-oxoethyl]-bis[(acetyloxy)methyl] ester (BAPTA-AM-Acetoxymethyl ester) (15551) (BAPTA-AM), and trans-resveratrol (70675) (RES) were purchased from Cayman Chemical (Ann Arbor, MI, USA). U-73122 (J62898) and 2-Aminoethyl diphenylborinate (A16606) (2-APB) were purchased from Alfa Aesar (Ward Hill, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (AC158990050) (MTT) was purchased from Acros Organics (Morris Plains, NJ, USA). 4′-Butyrate resveratrol (BuRV), 4′-Isobutyrate resveratrol (IsoRV) and 4′-Pivalate resveratrol (PIV) were synthesized by the Andrus lab in the Department of Chemistry and Biochemistry at Brigham Young University [18 (link)].
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2

Prostate Cancer Cell Line Characterization

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The PC-3 prostate cancer cell line was purchased from ATCC (Manassas, VA, USA). The passage numbers of cells used in experiments ranged from 5 to 20. Thapsigargin (10522) (TG), fura-2-acetoxymethyl ester (14591) (Fura-2), and trans-resveratrol (70675) (RES) were purchased from Cayman Chemical (Ann Arbor, MI, USA). U-73122 (J62898) and 2-Aminoethyl diphenylborinate (A16606) (2-APB) were purchased from Alfa Aesar (Ward Hill, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (AC158990050) (MTT) was purchased from Acros Organics (Morris Plains, NJ, USA). 4′-Butyrate resveratrol (BuRV), 4′-isobutyrate resveratrol (IsoRV) and 4′-pivalate resveratrol (PIV, Fig. 1) were synthesized by the Andrus lab in the Department of Chemistry and Biochemistry at Brigham Young University [29 (link)]. The purity of the compounds was determined to be greater than 98% by NMR and mass spectrometry.

Structures.

Structures of (A) trans-resveratrol, (B) 4′-pivalate trans-resveratrol, (C) 4′-isobutyrate trans-resveratrol, and (D) 4′-butyrate trans-resveratrol.

Fig. 1
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