3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt

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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used in colorimetric assays to measure cell viability and proliferation. It is a yellow tetrazolium salt that can be reduced to purple formazan crystals by metabolically active cells.

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Lab products found in correlation

Random hexamer primers, by NZYTech (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Oil red o staining, by Merck Group (1 mentions) Nzy m mulv reverse transcriptase, by NZYTech (1 mentions) Dapagliflozin, by Santa Cruz Biotechnology (1 mentions) Dntps, by NZYTech (1 mentions) Bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Gelatin sepharose 4b beads, by GE Healthcare (1 mentions) Costar transwell plate, by Corning (1 mentions)

2 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt

Cellular Assay Reagents Procurement

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Dapagliflozin and exenatide were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Fetal bovine serum (FBS) was purchased from Biochorm AG (Berlin, Germany). Insulin–transferrin–sodium selenite (ITS) supplement and gentamicin were obtained from Life Technologies (Carlsbad, CA, USA). Testosterone was purchased from Honeywell Fluka (Charlotte, NC, USA). Sulforhodamine B (SRB) was purchased from Biotum (Hayward, CA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was obtained from Amresco (Parkway Solon, OH, USA). All reagents for polymerase chain reaction (PCR) were purchased from NZYTech (Lisbon, Portugal), including: NZY M-MuLV Reverse Transcriptase, random hexamer primers, NZTaq 2x Green Master Mix, dNTPs, reaction buffer, and Greensafe. The lactate dehydrogenase (LDH)-Cytox^TM Assay Kit was purchased from BioLegend (San Diego, CL, USA). The bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Waltham, MA, USA). Tetraethylbenzimidazolylcarbocyanine iodide (JC-1) dye was purchased from Life Technologies (Gaithersburg, MD, USA). Oil Red O staining and other chemicals were all purchased from Sigma-Aldrich (St. Louis, MO, USA) unless stated otherwise.

Ribeiro J.C., Martins A.D., Jarak I., Carvalho R.A., Alves M.G, & Oliveira P.F. (2022). Exenatide and Dapagliflozin Combination Enhances Sertoli Cell Secretion of Key Metabolites for Spermatogenesis. Biomedicines, 10(5), 1115.

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AMR-Me Cellular Assay Protocol

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AMR-Me has been synthesized following a proprietary method described elsewhere [35 ]. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) was purchased from Gibco Life Technologies Corporation (Grand Island, NY). FN (440 kDa), protease inhibitor cocktail tablets (complete, mini, and EDTA-free), were purchased from Roche (Mannheim, Germany). Gelatin sepharose 4B beads were purchased from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). 3-(4,5-Dimethylthiazol-2-yl)-2-5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Amresco (Solon, OH). All primary antibodies (monoclonal and polyclonal), secondary antibodies were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA). Super Signal West Femto Maximum sensitivity substrate for enhanced chemiluminiscence was purchased from Thermo Scientific (Rockford, IL). RNAqueous^R-4PCR (DNA-free^™ RNA isolation for RT-PCR) kit, RETROscript Kit (for RT-PCR) and Super Taq Plus polymerase was purchased from Ambion, Life Technologies, Grand Island, NY. Primers for NF-κB were synthesized by Integrated DNA Technologies, Inc. (Corallville, IA). Costar transwell plates were purchased from Corning Inc. (Corning, NY).

Sinha D., Dutta K., Ganguly K.K., Biswas J, & Bishayee A. (2014). A novel synthetic oleanane triterpenoid suppresses adhesion, migration and invasion of highly metastatic melanoma cells by modulating gelatinase signaling axis. Molecular carcinogenesis, 54(8), 654-667.

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