Anti casp9 antibody

Tissues were lysed with RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific, United States), and protein lysates were resolved by SDS-PAGE gels (Thermo Fisher Scientific, United States), blotted onto PVDF membranes (Roche, Switzerland) for analysis, and incubated overnight at 4°C with anti-CASP9 antibody (1:1,000 dilution; proteintech, China) and anti-ACTB antibody (1:2000 dilution; Cell Signaling Technology, United States). Data analysis was performed using Image J software.

The tissues were sectioned and embedded in paraffin. Sections were incubated overnight at 4°C with anti-CASP9 antibody (1:100 dilution; proteintech, China). Slides were washed with phosphate-buffered saline (PBS) and incubated with a goat anti-rabbit IgG secondary antibody conjugated with fluorescein isothiocyanate (ZSDB-BIO, China) for 30 min with washed slides. After washing with PBS, they were incubated with an antifade reagent (Invitrogen, United States). Staining was visualized to determine protein expression levels using an Olympus CX41 fluorescence microscope (Olympus, Japan). The analysis results were performed using Image J software.