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5 protocols using β cyclodextrin β cd

1

Fluorescent Labeling of Cellular Components

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β‐Cyclodextrin (β‐CD) (CAS# 7585‐39‐9, Macklin), isopropanol (CAS# 67‐63‐0, Macklin), ethanol (CAS# 64‐17‐5, Macklin) were purchased from Shanghai McLean Biochemical Technology Co., Ltd. Methyl salicylate (MeSA) (CAS# 119‐36‐8, Leyan) was from Shanghai Haohong Biomedical Technology Co., Ltd. Fluorescein Isothiocyanate (FITC) (CAS# 3326‐32‐7, Coolaber), 1,1′‐Dioctadecyl‐3,3,3′,3′‐Tetramethylindodicarbocyanine, 4‐Chlorobenzenesulfonate Salt (DiIC18(5), DiD) (CAS# 127274‐91‐3, Coolaber) were obtained from Shenzhen Wenle Biotechnology Co., Ltd. 1,1 ‘‐ octadecyl‐3,3,3′, 3′‐tetramethylcyanamide perchlorate (DilC18(3), Dil) (CAS# 41085‐99‐8, Cayman) was purchased Beijing Biotech Co., Ltd. Lidocaine hydrochloride (CAS# 73‐78‐9, Aladdin) and sodium iodoacetate (CAS# 305‐53‐3, Aladdin) were from Shanghai Aladdin Biochemical Technology Co., Ltd. Triamcinolone acetonide (CAS# 76‐25‐5, Solarbio), 3,3′‐ dioctadecyl oxycarbocyanine perchlorate (DiOC18(3)) (CAS# 34215‐57‐1, Solarbio), Hoechst 33 342 (CAS# 875756‐97‐1, Solarbio), and Trizol (Invitrogen) were from Beijing Solarbio Technology Co., Ltd. Acupuncture needle (Hwato, 13 mm length, 0.25 mm diameter) was purchased from Suzhou Medical Supplies Factory Co., Ltd. Recombinant Anti‐CDC42 antibody [EPR15620] (ab187643) was obtained from Beijing Chengzhikewei Biotechnology Co., Ltd.
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2

Graphite-Cyclodextrin Composite Synthesis

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Graphite powder and β-cyclodextrin (β-CD) were purchased by Shanghai Macklin Biochemical Technology Co. Ltd. Ammonia solution were purchased from Tianjin Hengxing Chemical Reagent Factory. 4-Dimethylaminopyridine (DMAP), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and dopamine (DA) were produced by Aladdin Industrial Corporation of Shanghai. Except that the purity of ammonia water is 25%, other available reagents were of analytical grade without further purification. All solutions were prepared via ultra-pure water purified with the Millipore system from USA.
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3

Glucose, Phenylalanine, and Cyclodextrin Analysis

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D-glucose (Glu, 99.5%), L-phenylalanine (Phe, 99%), β-cyclodextrin (β-CD, 98%), sodium bisulfite (NaHSO3, AR), and formic acid (chromatographic grade) were purchased from Macklin (Shanghai, China). Ethanol, glycerol, and acetic acid were purchased from Damao Chemical Reagent Co., Ltd. (Tianjin, China). Methyl alcohol (chromatographic grade) was purchased from Merck (99.9%, Darmstadt, Germany). Ultrapure water (UPW) was used for all the experiments.
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4

PVDF Hollow Fiber UF Membrane Fabrication

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Polyvinylidene fluoride (PVDF) hollow fiber ultrafiltration (UF) membrane with MWCO of 30 K Da was purchased from Hangzhou Yuanxiang Film Co. Ltd (Hangzhou, China). The outer diameter of PVDF hollow fiber UF membrane is 1.4 mm. The pure water permeation (PWP) of PVDF hollow fiber UF membrane is 62 L·m−2·h−1·bar. PEI was purchased from Gobekie New Material Technology Co. Ltd (Shanghai, China). β-cyclodextrin (β-CD, 98%) and isophthaloyl dichloride (IPC, 98%) was obtained from Shanghai Macklin Biochemical Co. Ltd (Shanghai, China). n-hexane was purchased from Kermel Chemical Agent Co. Ltd (Tianjin, China). Bovine serum albumin (BSA, 99%) was purchased from Shanghai Rebiosci Biotechnology Co. Ltd (Shanghai, China). Inorganic salts, including magnesium chloride (MgCl2), sodium sulfate (Na2SO4), magnesium sulfate (MgSO4), were obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). All reagents were of analytical grade (AR) without further purification.
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5

Doxorubicin-Loaded Ti3AlC2 Nanoparticles

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Ti3AlC2, β-cyclodextrin (β-CD), hydrofluoric acid (HF), doxorubicin
(DOX), allyl bromide, p-toluenesulfonyl chloride (TsCl), ethylene
glycol, dimethylsulfoxide (DMSO), ferric chloride hexahydrate (FeCl3·6H2O), ammonium hydroxide (NH3·H2O, 25%, w/w), (3-mercaptopropyl) triethoxysilane
(MPTMS), and polyethylene glycol (PEG-2000, Mn = 2000) were obtained from Macklin Reagent (Shanghai, China).
Acetic acid, sodium chloride (NaCl), hydrochloric acid (HCl), sodium
acetate, sodium hydroxide (NaOH), phosphoric acid (H3PO4), nitric acid (HNO3), sodium hydrogen phosphate
(Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), and ethanol were purchased from Jiangsu Qiangsheng
Chemical Co., Ltd. (Changshu, China). The DOX stock solution (100
μg mL–1) was prepared by deionized water.
The grade of NdFeB strong magnet is N35, and the residual magnetic
flux density is 11,700–12,200 Gs.
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