Anrneasy mini kit

Manufactured by Qiagen

Sourced in United States, Germany

The AnRNeasy mini kit is a laboratory equipment designed for the extraction and purification of total RNA from a variety of sample types. It utilizes a silica-based membrane technology to efficiently capture and purify RNA molecules.

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Lab products found in correlation

Qiashredder column, by Qiagen (1 mentions) Accutase, by Merck Group (1 mentions) Ripa buffer, by Abcam (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Omniscript rt kit, by Qiagen (1 mentions) Precision nanoscript reverse transcription kit, by Primerdesign (1 mentions) Taqman universal master mix with uracil n glycosylase, by Thermo Fisher Scientific (1 mentions) Verso cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)

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Mini Kits (17 citations)

4 protocols using anrneasy mini kit

Conditioned Medium Effects on Glioblastoma Stem Cells

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750,000 PN GSCs were
seeded in 3 mL of GSC medium and treated with 3 mL of conditioned
medium (CM) obtained from MES GSCs grown for 4 days. Four days later,
cells were harvested, dissociated with Accutase (Sigma-Aldrich, A6964),
and washed with DPBS (Sigma-Aldrich, #D8537). Each sample was divided
into two parts for RNA and protein isolation. For EV treatment, 250,000
PN cells were incubated with NCH705-derived sEVs (9 μg protein
equivalent) for 4 days and harvested, dissociated, and washed for
protein extraction. To extract proteins, cell pellets were resuspended
in 50 μL of RIPA Buffer (Abcam, #ab156034) and incubated for
15 min on ice. Lysates were homogenized using QIAShredder columns
(Qiagen, #79656), and protein concentration was determined by BCA
assay (Thermo Fisher Scientific, #23225). RNA was isolated using an
RNeasy mini kit (Qiagen, #74104) according to the manufacturer’s
protocol.

Lokumcu T., Iskar M., Schneider M., Helm D., Klinke G., Schlicker L., Bethke F., Müller G., Richter K., Poschet G., Phillips E, & Goidts V. (2024). Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. ACS Nano, 18(3), 2500-2519.

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Quantitative Real-Time RT-PCR Analysis of EMT Markers

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Total RNA was extracted from cell lines using an-RNeasy Mini Kit^® (Qiagen, Valencia, CA, USA) and reverse transcribed at 37 °C for 60 min with an Omniscript RT Kit^® (Qiagen) according to the manufacturer’s instructions. Real-time RT-PCR analysis was performed in triplicate in a Step One Plus Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) with Power SYBR^® Green PCR Master Mix (Applied Biosystems) in a final volume of 20 μL/reaction. The threshold cycle (C_t) value of each tested gene was normalized to the C_t value of the GAPDH control from the same RNA preparation. The ratio of transcription of each gene was calculated as 2^−(ΔCt), where ΔC_t is the difference C_{t(test gene)} − C_t(GAPDH). The real-time RT-PCR primer sequences used in this study were: N-cadherin F-5′-ACAGTGGCCACCTACAAAGG-3′, R-5′-CCGAGATGGGGTTGATAATG-3′, E-cadherin F-5′-ACGTCGTAATCACCACACTGA-3′, R-5′-TTCGTCACTGCTACGTGTAGAA-3′, Twist F-5′-CGGGAGTCCGCAGTCTTA-3′, R-5′-TGAATCTTGCTCAGCTTGTC-3′, vimentin F-5′-TCTAC GAGGAGGAGATGCGG-3′, R-5′-GGTCAAGACGTGCCAGAGAC-3′, and GAPDH F-5′-CCATGG AGAAGGCTGGGG-3′, R-5′-CAAAGTTGTCATGGATGACC-3′.

Su Y.J., Huang S.Y., Ni Y.H., Liao K.F, & Chiu S.C. (2018). Anti-Tumor and Radiosensitization Effects of N-Butylidenephthalide on Human Breast Cancer Cells. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(2), 240.

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Real-Time PCR Quantification of Sgk1 and Sgk3 in Rodent Brains

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For rat brain lysates, total RNA was converted to cDNA using random primers and
Life Technologies Superscript II. The cDNA was diluted (5 ng per µl) and Taqman
reactions performed with 20 ng cDNA per well under standard conditions in 25 µl
reaction volume. Taqman probesets for rat Sgk1 and 18S ribosomal RNA were
obtained from Applied Biosystems, Paisley, UK (Rn00570285_m1 and #4310893E,
respectively). For mouse brain lysates, total RNA was isolated from cerebra of
young adult male mice from the Sgk1^+/+ and Sgk1^−/−colonies. Whole cerebral hemispheres were homogenized and passed through
QIAshredder™ columns (QIAGEN GmbH, Hilden, Germany). RNA was extracted using an
RNeasy® Mini kit (QIAGEN GmbH, Hilden, Germany). Total RNA (500 ng) was
converted to cDNA using a Precision Nanoscript reverse transcription kit with
random primers (Primerdesign Ltd, Southampton, UK). Amplification reactions were
performed in a 20-µl reaction volume containing 250 ng cDNA for Sgk3 and Sgk1
assays, and 25 ng cDNA for the housekeeping gene Gapdh, using Taqman® Universal
mastermix with uracil-N-glycosylase (Applied Biosystems, Paisley, UK). Taqman®
expression assays for mouse Sgk3 (assay # Mm01227735_m1), Sgk1 (Mm00441387_g1)
and gapdh (Mm99999915_g1) were purchased from Applied Biosystems-Life
Technologies, Paisley, UK.

McCaig C., Ataliotis P., Shtaya A., Omar A.S., Green A.R., Kind C.N., Pereira A.C., Naray-Fejes-Toth A., Fejes-Toth G., Yáñez-Muñoz R.J., Murray J.T, & Hainsworth A.H. (2017). Induction of the cell survival kinase Sgk1: A possible novel mechanism for α-phenyl-N-tert-butyl nitrone in experimental stroke. Journal of Cerebral Blood Flow & Metabolism, 39(6), 1111-1121.

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Quantitative Analysis of Osteoclast Genes

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BMMs were seeded
in a 6-well plate (3 × 10⁵ cells/well). After four
days of induction with MCSF and RANKL along with TRPV1 agonists and
antagonists, the cells were processed for RNA extraction using an
Rneasy mini kit (QIAGEN). The cDNA was synthesized using a Verso cDNA
synthesis kit (Thermo Scientific). cDNA from various samples were
diluted to 5 ng/μL concentration, and a total of 25 ng of DNA
was used for qRT-PCR reactions, set up in a 96-well plate sealed with
an optical film (Thermo Fisher). For each sample, reactions were set
in triplicates. SYBR green chemistry was employed to perform quantitative
detection of the relative expression of mRNA levels of these genes
using an ABI 7500 RT-PCR system.

Chakraborty R., Acharya T.K., Tiwari N., Majhi R.K., Kumar S., Goswami L, & Goswami C. (2022). Hydrogel-Mediated Release of TRPV1 Modulators to Fine Tune Osteoclastogenesis. ACS Omega, 7(11), 9537-9550.

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