Horseradish peroxidase hrp conjugated anti mouse or anti rabbit goat secondary antibodies

McCoy’s 5A growth medium, phosphate buffered saline (PBS), and trypan blue were purchased from ATCC (Manassas, VA). Fetal bovine serum (FBS), penicillin/streptomycin, and 0.25% trypsin-EDTA (w/v) were purchased from Gibco (Grand Island, NY). Arsenic trioxide (lot # 02765-24) and Nunc TM Lab-TekTM chamber slides were purchased from Fisher Scientific (Houston, TX). A lipid peroxidation kit was purchased from Abcam (Cambridge, MA). Prolong Gold Antifade Reagent with 4′,6-diamidine phenylindone (DAPI) solution was purchased from Invitrogen Corporation (Grand Island, NY). ECL western blotting detection system reagents and Films (CL-X-posure) were purchased from Thermo Scientific/Pierce (Rockford, IL). Pre-cast gels were purchased from Fisher Scientific (Pittsburg, PA). The folin-phenol (DC) protein determination kit, cell lysis buffer, and non-fat milk were obtained from BioRad Laboratories (Hercules, CA) and, polyvinylidene difluoride (PVDF) membrane was obtained from Millipore (Bedford, MA). The primary antibodies anti-caspase 3 and anti-cytochrome c were purchased from Cell Signal Technology, Inc (Danvers, MA). The primary antibodies anti-Bcl-2, anti-Bax, and horseradish peroxidase (HRP) conjugated anti-mouse or anti-rabbit goat secondary antibodies and β-actin were purchased from EMD Millipore Corporation (Billerica, MA).