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Anti cd301 lom 14

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Anti-CD301 (LOM-14) is a lab equipment product used for the detection and analysis of CD301, a cell surface receptor expressed on certain immune cells. It is designed for research purposes only and its core function is to enable the identification and characterization of CD301-positive cells in various experimental settings.

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Lab products found in correlation

Normal mouse serum, by Jackson ImmunoResearch (2 mentions) Anti cd11b m1 70, by Thermo Fisher Scientific (2 mentions) Tcs sp5, by Leica (2 mentions) Aqua poly mount, by Polysciences (2 mentions) Anti cd4 rm4 5, by BioLegend (2 mentions)

Close spelling variants of this product

Rat monoclonal anti-CD301 (Clone LOM-14, CD301

2 protocols using anti cd301 lom 14

1

Adipose Tissue Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
For detection of GFP and PBS57-CD1d tetramer staining, epididymal or subcutaneous adipose tissue was harvested into PBS/0.02% sodium azide /5% normal mouse serum (Jackson ImmunoResearch Laboratories, Inc.). Tissues were first stained with PBS57 loaded-CD1d tetramers (provided by the NIH Tetramer Core Facility) at room temperature for 60 min in the dark on a circular shaker. Subsequent antibody staining was performed on ice for 60 min, in the dark on a circular shaker. Next, tissue was washed with PBS/ 0.02% sodium azide/ 5% mouse serum. Adipose tissue was imaged on glass slides with Aqua Poly/Mount (Polysciences, Inc.) and a coverslip by confocal microscopy (Leica TCS SP5). Surface antibody staining included anti-CD11b (M1/70) from eBioscience, anti-CD4 (RM4-5) and anti-CD301 (LOM-14) from Biolegend.
Lynch L., Michelet X., Zhang S., Brennan P.J., Moseman A., Lester C., Besra G., Vomhof-Dekrey E.E., Tighe M., Koay H.F., Godfrey D.I., Leadbetter E.A., Sant’Angelo D.B., von Andrian U, & Brenner M.B. (2014). Regulatory iNKT cells lack PLZF expression and control Treg cell and macrophage homeostasis in adipose tissue. Nature immunology, 16(1), 85-95.
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2

Adipose Tissue Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
For detection of GFP and PBS57-CD1d tetramer staining, epididymal or subcutaneous adipose tissue was harvested into PBS/0.02% sodium azide /5% normal mouse serum (Jackson ImmunoResearch Laboratories, Inc.). Tissues were first stained with PBS57 loaded-CD1d tetramers (provided by the NIH Tetramer Core Facility) at room temperature for 60 min in the dark on a circular shaker. Subsequent antibody staining was performed on ice for 60 min, in the dark on a circular shaker. Next, tissue was washed with PBS/ 0.02% sodium azide/ 5% mouse serum. Adipose tissue was imaged on glass slides with Aqua Poly/Mount (Polysciences, Inc.) and a coverslip by confocal microscopy (Leica TCS SP5). Surface antibody staining included anti-CD11b (M1/70) from eBioscience, anti-CD4 (RM4-5) and anti-CD301 (LOM-14) from Biolegend.
Lynch L., Michelet X., Zhang S., Brennan P.J., Moseman A., Lester C., Besra G., Vomhof-Dekrey E.E., Tighe M., Koay H.F., Godfrey D.I., Leadbetter E.A., Sant’Angelo D.B., von Andrian U, & Brenner M.B. (2014). Regulatory iNKT cells lack PLZF expression and control Treg cell and macrophage homeostasis in adipose tissue. Nature immunology, 16(1), 85-95.
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