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4 protocols using calcium chloride

1

Synthesis of Inorganic Compounds

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The AR grade chemicals, namely, lead nitrate, sodium alginate, calcium chloride, nitric acid, hydrochloric acid, and sodium hydroxide, were purchased from SD Fine Chemicals Pvt. Ltd. and Merck & Co. Double distilled water was used for preparation of solutions.
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2

Synthesis of SiO2 Nanoparticles

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Sodium alginate and gelatin purchased from HIMEDIA, Hyderabad, India. Calcium chloride was obtained from SD Fine Chem. Limited, India. 10× PBS (Sigma Aldrich, India) was diluted to 1× PBS and used in experiments. For the synthesis of SiO2 nanoparticles, reagents like tetraethyl orthosilicate (TEOS, 99%, Alfa Aesar), ethanol (99.98%, Pharmco-Aaper) and ammonia (30%, Sisco Research Laboratories) were used.
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3

Fabrication of Polymeric Drug Delivery Devices

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Meloxicam was acquired from Glenmark Pharma private Ltd, Mumbai, India. All the polymers received were of pharmaceutical grade (Chitosan/ HPMCK4/HPMCK15 are obtained from Lepid Life Sciences Pvt Ltd, Delhi, India and Guar gum/Gellan gum/ Xantha gum/pectins are obtained from Sigma-Aldrich Germany) were used as received. Sodium alginate, Sodium citrate and Calcium chloride were obtained from S.D Fine chemicals, Mumbai, India. All chemicals and solvents utilized were of HPLC grade. Throughout the study distilled water was used.
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4

Rectal Mucosa Permeation Study

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OXC was kindly donated by Sun Pharma, Baroda (Gujarat, India). Carbopol 971 P was gifted by Noveon Pharmaceuticals Private Limited, Cleveland (USA). Poloxamer 188 was donated by Mylan Laboratories (Nasik, India). Sodium alginate was gifted by Thomas Baker Chemicals Private Limited, Mumbai, India. Liquid paraffin, potassium dihydrogen phosphate, calcium chloride, sodium hydroxide and propylene glycol were obtained from SD Fine Chemicals, Mumbai (Maharashtra, India). Methanol and acetonitrile were procured from Rankem Limited, India. Freshly excised rectal mucosa of goat was obtained from a local butcher (Nasik, India). All the other chemicals and reagents were of analytical grade and were used without further purification.
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