Meohp

A total of 11 phthalate metabolites were measured in the patient urine samples, as stated above.20 (link) The phthalate metabolites comprised monomethyl phthalate (MMP), MEP, mono-n-butyl phthalate (MBP), monocyclohexyl phthalate (MCHP), MBzP, MiBP, mono-2-ethylhexyl phthalate (MEHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono(2-ethyl-5-carboxypentyl) phthalate (MECPP), monooctyl phthalate (MOP), and monoisodecyl phthalate (MiNP).
The chemical urine sample analyses were conducted at the Fudan University of Public Health laboratory. The phthalates in the urine were analyzed via liquid chromatography–mass spectrometry–tandem mass spectrometry (American Agilent company) with ultra-performance liquid chromatography coupled with a tandem mass spectrometer and Quattro Premier XE following enzymatic deconjugation.20 (link)
Standards: MMP, MBP, MEHP, MNzP (chromatographically pure, Dr. Ehrenstorfer, Germany), MCHP, MEOHP, MiBP, MOP, MiNP (chromatographically pure, Toronto Research Chemicals, Canada), MEP (chromatographically pure, AccuStandard, USA), MECPP (chromatographically pure, Cambridge Isotope Laboratories, Inc., USA), internal standard (chromatographically pure, Cambridge Isotope Laboratories, Inc., USA), and β-glucuronidase (>99.99%, Merck Millipore, Germany). Other reagents are HPLC grade or pesticide residue grade.

Phthalate metabolites, mEHP, mEHHP, mEOHP, mnBP, miBP and mBzP were purchased at 98% purity from Toronto Research Chemicals (TRC Inc). Most of them were solids except for mEHHP and mEOHP which were oils. Methanol, ethyl acetate and acetonitrile were Chromasolv grade for HPLC and purchased from Sigma–Aldrich (St Louis, MO, USA). Phenobarbital-d5, used as internal standard was obtained from Isotec Inc (Miamisburg OH, USA). Ultrapure water was produced by a Direct-Q 3UV water purification system (Merck, Germany). Escherichia Coli (E. Coli) β-glucuronidase K12 (140 units/mg, 5 ml), was purchased from Sigma-Aldrich. Amniotic fluid was collected with glass syringes 10 ml FORTUNA OPTIMA (Germany) and it was stored in amber glass bottles 5 ml (Sigma-Aldrich). SPE Cartridges C18 (100 mg) were obtained from Sigma-Aldrich.