Hle b3

HLE-B3 cells were obtained from the American Type Culture Collection. HLE-B3 cells were cultured in minimum essential medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin, and maintained in a humidified incubator with 5% CO₂ at 37˚C.
Oxidants induce cell apoptosis and trigger the development of cataracts (10 (link)). As peroxidative damage is mediated by the toxic metabolites of oxygen, such as hydroxide, H₂O₂ is frequently used to induce the apoptosis of LECs in vitro. For the establishment of an in vitro cataract model, HLE-B3 cells (1x10⁶ cells/well) were seeded into 6-well plates and induced at 37˚C with 200 µmol/l H₂O₂ (Sigma-Aldrich; Merck KGaA) for 24 h, as previously described (17 (link),18 (link)), while cells in control group were untreated.

The Human lens epithelial cell (HLE-B3) cell line was from the American Type Culture Collection (ATCC, VA, USA), HLE-B3 cells were cultured in a humidified 5% CO₂ incubator at 37 °C with MEM (Gibco, Carlsbad, CA, USA) containing 1% PS and 10% FBS.