Elia gliadindp igg

Detection of IgA anti-TG2, IgA anti-GL, and Immunoglobulin G (IgG) anti-DGP in serum or plasma was performed using EliA Celikey IgA (positive result ≥ 7 U/mL), EliA Gliadin IgA (positive result ≥ 7 U/mL), and EliA GliadinDP IgG (positive result ≥ 10 U/mL), respectively, on Phadia250 (Thermo Fisher Scientific, Waltham, MA) as described by the manufacturer. In cases with total IgA levels below 0.07 g/L, detection of IgG anti-TG2 replaced IgA anti-TG2 (EliA Celikey IgG, Thermo Fisher Scientific). In order to distinguish results below the detection limit of an assay from missing data, the former were replaced with the detection limit divided by two.

The levels of serum IgA autoantibodies against TG2 were assessed with anti-TG2 ELiA (Thermo Science, 14-5517-01) with manufacturer’s cut off value of > 10 U/ml. Cut off values of anti-dGP IgG and IgA are at > 10 U/ml (Thermo Science, ELiA GliadinDP IgG, 14-5538-01; ELiA GliadinDP IgA, 14-5539-01). All measurements were made using the programmable ELISA reader Phadia™ 250.