Dm1000 led light microscope

ACL tissue samples (length: ~2 mm) were fixed overnight in 4% PFA solution, dehydrated and finally embedded in paraffin. After slicing, the 7 µm sections were rehydrated in a descending alcohol series and prepared for hematoxylin eosin (HE) and alcian blue (AB) staining.
For HE staining paraffin sections and glass cover slips were incubated for 6 min in Harry’s hematoxylin (Sigma-Aldrich, Munich, Germany), before rinsed in tap water and counterstained for 4 min in eosin (Carl Roth GmbH, Karlsruhe, Germany).
For AB performance, the tissue sections were rehydrated in a descending alcohol series and subsequently incubated for 3 min in 1% acetic acid before incubated for 30 min in 1% AB staining solution (Carl Roth GmbH, Karlsruhe, Germany). After rinsing in 3% acetic acid and a 2 min washing step in A. dest., ligamentocyte cell nuclei were counterstained for 5 min in nuclear fast red aluminium sulphate solution (Carl Roth GmbH).
HE and AB stained sections were covered with Entellan (Merck-Millipore, Darmstadt, Germany). Photos were taken using a DM1000 LED light microscope (Leica, Wetzlar, Germany).

Representative tissue samples of three cadavers (sex: all male, age: 68, 69 and 84 years) were fixed in 4% neutral buffered paraformaldehyde solution, dehydrated and paraffin embedded and sectioned at 7 µm according to a standard protocol. Horizontal sections were performed. These samples were then stained with hematoxylin and eosin (H&E) (Sigma-Aldrich, Munich, Germany). A DM1000 LED light microscope (Leica, Wetzlar, Germany) was used to take photos.