Testicular tissue sections from IV:3, which is a patient with infertility belonging to the family (figure 1A ), WT mice and Xrcc2L14/L14P mice were immunereacted with a primary anti-XRCC2 antibody (1:100 dilution of rabbit polyclonal antibody, Gene Tex,GTX16397) to assess the XRCC2 expression in the testes. The test of affinity of the XRCC2 antibody (Gene Tex, GTX16397) was performed by over expression assay (online supplementary figure S1 ).
The primary antibody was detected using an HRP-labelled goat anti-rabbit IgG polymer (PV-6001, ZSGB-BIO, China). HRP interacting with hydrogen peroxide and dark brown DAB was visualised on a microscope in accordance with the product protocol.
The primary antibody was detected using an HRP-labelled goat anti-rabbit IgG polymer (PV-6001, ZSGB-BIO, China). HRP interacting with hydrogen peroxide and dark brown DAB was visualised on a microscope in accordance with the product protocol.