Navious flow cytometer

Manufactured by Beckman Coulter

Sourced in United States

The Navios flow cytometer is an analytical instrument designed for the measurement and analysis of cells and particles in a fluid sample. It utilizes laser technology to detect and characterize the properties of individual cells as they pass through a laser beam. The Navios collects data on various parameters, such as size, granularity, and fluorescence, which can be used to identify and quantify different cell populations. This instrument is commonly used in clinical and research applications for tasks such as immunophenotyping, cell sorting, and the assessment of cellular function.

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Lab products found in correlation

Pe conjugated anti cd11b, by BD (1 mentions) Fitc conjugated anti cd206, by BioLegend (1 mentions) Rnase a, by Merck Group (1 mentions) Kaluza software, by Beckman Coulter (1 mentions)

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Navious

3 protocols using navious flow cytometer

Characterizing Lung Immune Cells

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After blocking the Fc receptors, isolated BALF lung cells were washed and
resuspended in PBS supplemented with 1% fetal bovine serum. Cells were stained
with FITC-conjugated anti-CD206 (BioLegend, San Diego, CA, USA), PE-conjugated
anti-CD11b, and PE-conjugated anti-major histocompatibility complex (MHC) class
II (BD Biosciences, San Jose, CA, USA) for 30 minutes at 4°C.^{10 (link),11} Flow
cytometry was then performed using a Navious flow cytometer (Beckman Coulter,
Miami, FL, USA) for quantification.

Jo W.S., Kang S., Jeong S.K., Bae M.J., Lee C.G., Son Y., Lee H.J., Jeong M.H., Kim S.H., Moon C., Shin I.S, & Kim J.S. (2022). Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model. Dose-Response, 20(3), 15593258221117349.

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Cell Cycle Analysis by Flow Cytometry

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Cell cycle analysis was performed as described earlier^{48 (link)}, briefly, cell pellets were washed in PBS and re-suspended and stained in 500 μl PBS supplemented 100 μg/ml RNAse A (Sigma, St. Louis, MO, USA) and 50 μg/ml of Propidium Iodine and were analyzed by Navious flow cytometer (Beckman Coulter, Miami, Florida, USA). Staining was detected in the fluorescence channel (FL3) and the data were analyzed using Kaluza software (Beckman Coulter).

Vishnubalaji R., Elango R., Manikandan M., Siyal A.A., Ali D., Al-Rikabi A., Hamam D., Hamam R., Benabdelkamel H., Masood A., Alanazi I.O., Alfadda A.A., Alfayez M., Aldahmash A., Kassem M, & Alajez N.M. (2020). MicroRNA-3148 acts as molecular switch promoting malignant transformation and adipocytic differentiation of immortalized human bone marrow stromal cells via direct targeting of the SMAD2/TGFβ pathway. Cell Death Discovery, 6, 79.

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Comprehensive Flow Cytometry Analysis

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CSF and blood samples were analyzed in the centralized CSF laboratory of the University Clinic Münster within 1 h of sample taking. Standardized mFC of CSF and blood samples using a predefined staining panel are routinely performed at our center of all CSF samples collected during regular working hours. Analysis was performed once per sample.
Samples were centrifuged for 15 min at 300 × g, supernatant was removed, and staining was performed based on an established protocol [37 (link)]. The following antibodies were used: CD3 (UCHT1); CD4 (13B8.2); CD8 (B9.11); CD14 (RMO52); CD16 (3G8); CD19 (J3–119); CD45 (J.33); CD56 (C218); CD138 (B-A38); HLA-DR (Immu-357) (all Beckman Coulter, clones in brackets). Gating was performed on forward scatter and sideward scatter and afterward on CD45+ cells. Percentage of cell population was analyzed as described before [30 (link)]. Analysis was performed using a Navious flow cytometer (Beckmann Coulter) and the software Kaluza (version 2.1).

Räuber S., Heming M., Repple J., Ruland T., Kuelby R., Schulte-Mecklenbeck A., Gross C.C., Arolt V., Baune B., Hahn T., Dannlowski U., Meuth S.G., Melzer N., Wiendl H, & Meyer zu Hörste G. (2021). Cerebrospinal fluid flow cytometry distinguishes psychosis spectrum disorders from differential diagnoses. Molecular Psychiatry, 26(12), 7661-7670.

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