miR-23a mimics (miR-23a), control mimics (miR-control), siRNA against GAS5 (si-GAS5), siRNA control (si-control), pcDNA-GAS5, and pcDNA empty control (pcDNA-control) were purchased from GenePharma (Shanghai, P.R. China). Cultured A549 and H157 cells were transfected with miR-23a, si-GAS5, pcDNA-GAS5, pcDNA-GAS5 + miR-23a, or their corresponding control at approximately 60%–70% confluence using Lipofectamine 2000 (Invitrogen). Transfection efficiency was checked by quantitative real-time polymerase chain reaction (qRT-PCR). Subsequent experiments were performed 48 h after transfection.
Pcdna gas5
Manufactured by GenePharma
Sourced in China
PcDNA-GAS5 is a plasmid vector used for gene expression studies. It contains the GAS5 gene, which encodes a long non-coding RNA involved in various cellular processes. The plasmid can be used for transfection experiments in cell culture systems to investigate the function and regulation of the GAS5 gene.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Mir control, by GenePharma (1 mentions)
Mir 23a, by GenePharma (1 mentions)
Pcdna gas5, by Thermo Fisher Scientific (1 mentions)
Sirna control si control, by GenePharma (1 mentions)
Pcdna control, by GenePharma (1 mentions)
Si gas5, by GenePharma (1 mentions)
Si pten, by GenePharma (1 mentions)
Si ezh2, by GenePharma (1 mentions)
Pcdna3, by Vector Laboratories (1 mentions)
Si hoxd as1, by GenePharma (1 mentions)
Si con, by GenePharma (1 mentions)
2 protocols using pcdna gas5
1
Regulation of GAS5 by miR-23a in Lung Cancer
2
Overexpression and Knockdown of GAS5, EZH2, and PTEN
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The empty vector pcDNA3.1 (Vector) or GAS5 overexpressing vector pcDNA3.1-HOXD-AS1 (pcDNA-GAS5) and small interfering RNAs against GAS5 (si-HOXD-AS1), EZH2 (si-EZH2) or PTEN (si-PTEN) or their scramble negative siRNA (si-con) were obtained from Genepharma (Shanghai, China). The Lipofectamine 2000 (Invitrogen) was used to perform cell transfections.
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