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Low speed diamond blade saw

Manufactured by Buehler
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The Low Speed Diamond Blade Saw is a versatile laboratory equipment designed for precision cutting of a variety of materials, including metals, ceramics, and composites. This saw utilizes a diamond-tipped blade that rotates at a low speed, enabling controlled and accurate cuts. Its core function is to provide a reliable and consistent cutting solution for sample preparation and analysis in various research and testing applications.

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Low speed saw (8 citations) Low-speed diamond saw (4 citations) Diamond blade (3 citations) Diamond blade saw (2 citations) Diamond saw (2 citations)

3 protocols using low speed diamond blade saw

1

Dental Tissue Specimen Preparation

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Specimens of dental tissues were prepared using a protocol modified slightly from our previous publications [45 (link)]. The specimens consisted of extracted human molars. These teeth would otherwise be discarded, no patient identifiers are associated with the teeth and thus, this is not considered human subject research. The extracted teeth were stored at 4 °C in 0.9% wt/vol NaCl containing 0.002% sodium azide. Teeth exhibiting carious lesions were selected and the root structure was removed by sectioning perpendicular to the long axis of the molar using a water-cooled low-speed diamond-blade saw (Buehler, Lake Bluff, IL, USA). The tooth was sectioned parallel to the long axis to provide 1.4 mm thick slabs. The prepared sections were stored in a modified PBS buffer (20 mM Na2HPO4, 137 mM NaCl, 2.7 mM KCl, and 1.8 mM KH2PO4, pH 7.4) at 4 °C until experimental use.
Woolfolk S.K., Cloyd A.K., Ye Q., Boone K., Spencer P., Snead M.L, & Tamerler C. (2022). Peptide-Enabled Nanocomposites Offer Biomimetic Reconstruction of Silver Diamine Fluoride-Treated Dental Tissues. Polymers, 14(7), 1368.
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2

Preparation of Bovine Femur Bone Samples

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Five mature bovine femurs were obtained from a local slaughterhouse and cleaned of soft tissue. Femurs were wrapped in water soaked tissue paper, vacuum sealed into plastic bags and stored frozen at −20° C until used. Cortical bone samples were cut longitudinally from the four quadrants of the mid-diaphysis of bovine femurs using a low speed diamond blade saw (Buehler Ltd., Lake Bluff, IL). Samples were then progressively polished (Buehler Ltd., Lake Bluff, IL) with 600, 800, 1200 grade polishing paper and fine alumina powder (0.3 μm). Polishing and cutting debris was removed by sonication (Model 8890, Cole-Parmer, Vernon Hills, IL). The final dimensions of bone beams (N=30) were 30 mm in length, 3 mm in width and 1.1 mm in thickness. Samples were stored wrapped in distilled water soaked Kimwipes and stored at −20 °C. Samples were thawed completely prior to the analyses.
Unal M, & Akkus O. (2015). RAMAN SPECTRAL CLASSIFICATION OF MINERAL- AND COLLAGEN-BOUND WATER’S ASSOCIATIONS TO ELASTIC AND POST-YIELD MECHANICAL PROPERTIES OF CORTICAL BONE. Bone, 81, 315-326.
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3

Implant Retrieval and Histological Analysis

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Fifteen and thirty days after implantation, six rats with control and anodised implants, Zr30and Zr60V (all individuals from different litters) were deeply anaesthetised with Ketamine/Xylasine (100 mg/kg, 10 mg/kg) and sacrificed with an overdose of pentobarbital. The retrieved samples were cleaned from surrounding soft tissues and fixed in neutral 10 wt% formaldehyde for 24 h. Then they were dehydrated in a series of alcohol-water mixtures followed by a methacrylated solution and finally embedded in a polymethyl methacrylate (PMMA) solution and polymerized. The PMMA embedded blocks were cut with a low speed diamond blade saw (Buehler GmbH) cooled with water. Sections were made 100 μm thick and polished to a final thickness of about 60 μm for histological observations.
Katunar M.R., Gomez Sanchez A., Santos Coquillat A., Civantos A., Martinez Campos E., Ballarre J., Vico T., Baca M., Ramos V, & Cere S. (2017). In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications. Materials science & engineering. C, Materials for biological applications, 75.
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