Leitz dmrb fluorescence microscope

Manufactured by Leica

The Leitz DMRB is a fluorescence microscope designed for advanced microscopy applications. It features a robust construction and provides high-quality imaging capabilities. The core function of the Leitz DMRB is to enable fluorescence imaging and analysis of biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Lsm780 confocal laser scanning microscope, by Zeiss (1 mentions) Poly l lysine coated slides, by Avantor (1 mentions)

Spelling variants of this product

Fluorescence microscope (2465 citations) DMRB microscope (189 citations) DMRB fluorescence microscope (39 citations) Leitz DMRB microscope (29 citations) Leitz DMRB (18 citations) Leitz fluorescence microscope (2 citations)

3 protocols using leitz dmrb fluorescence microscope

Quantification of Plasmodium berghei Merozoites and Growth Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to evaluate the number of merozoites per schizont, purified schizonts from P. berghei cultures were fixed in 4% paraformaldehyde and 0.0075% glutaraldehyde for 10 min at 4°C then incubated 30 min with DAPI. The nuclei were counted with Leica Leitz DMRB fluorescence microscope. To measure growth rate, purified mature schizonts were intravenously injected in CD1 mice. Smears were performed at 1h, 22h, 25h and 29h post-infection and rings, trophozoites and schizonts were counted under the microscope after Giemsa staining.
For sexual development, the gametocytemia was determined by microscopy. Exflagellation was assessed after 10–12 min of incubation at 21°C in RPMI 1640 with 25 mM HEPES and 10% fetal calf serum, pH 8 [75 ]. The exflagellation centers were counted under slide-coverslip by microscopy using a 40x objective.

Hollin T., De Witte C., Fréville A., Guerrera I.C., Chhuon C., Saliou J.M., Herbert F., Pierrot C, & Khalife J. (2019). Essential role of GEXP15, a specific Protein Phosphatase type 1 partner, in Plasmodium berghei in asexual erythrocytic proliferation and transmission. PLoS Pathogens, 15(7), e1007973.

+ Open protocol

+ Expand

Fluorescence Imaging and Colocalization Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescent images were acquired using a Carl Zeiss LSM 780 LASER scanning confocal microscope (Montpellier RIO Imaging facility) and a 40× PLAN Apochromatic 1.3 oil immersion objective. The acquisition software was Zen. Contrast and relative intensities were processed with ImageJ software. Light microscope images were acquired using Leica Leitz DMRB Fluorescence Microscope with Nomarsky lens. Colocalization was quantified by FIJI as follows: Background was subtracted with a rolling ball radius of 30 µm, and the Pearson correlation coefficient (PCC) was calculated using the colloc2 plugin with auto-thresholding. Mean PCC was calculated from three images.

Götze M., Dufourt J., Ihling C., Rammelt C., Pierson S., Sambrani N., Temme C., Sinz A., Simonelig M, & Wahle E. (2017). Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch. RNA, 23(10), 1552-1568.

+ Open protocol

+ Expand

Sperm Isolation and Immunocytochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Semen samples (N, S and P) were thawed and incubated at 37°C for at least 30 min prior to centrifugation on a 60% cushion of Puresperm equilibrated with PureCeption Isotonic Solution (SAGEMedia, UK) mixed with Quinn's Sperm Washing Medium (SAGE, UK) at 400 × g for 20 min. The 60% DGC layers and the pellets were collected and washed twice with Quinn's Sperm Washing Medium at 500xg for 10 min.
For immunocytochemistry, cells recovered from 60% cushions were cytospun at 500 × g for 15 minutes on to poly-l-lysine coated slides (VWR, UK). Cells were fixed in 3:1 methanol/acetone for 15 min, washed with Phosphate Buffered Saline (PBS) and incubated in blocking solution (2% goat serum, 2% serum albumin, 0.1% Triton X-100 in PBS) for 30 min at room temperature (RT). Antibodies were applied to slides in a humidified chamber for 60 min at RT (Supplementary Table 3). Slides were subsequently washed in PBS and incubated for 30 min with appropriate fluorescence-conjugated secondary antibodies in blocking solution at RT. Slides were finally washed in PBS and mounted with an antifade-containing polyvinyl alcohol-mounting medium (DABCO, UK). Fluorescence microscopy was undertaken with a Leica LEITZ DMRB fluorescence microscope. The images were taken with a cooled CCD camera (Orca-03 R, Hamamatsu) controlled by Smart Capture 3 software (DSUK, UK).

Lorente G., Ntostis P., Maitland N., Mengual L., Musquera M., Muneer A., Oliva R., Iles D, & Miller D. (2021). Semen sampling as a simple, noninvasive surrogate for prostate health screening. Systems biology in reproductive medicine, 67(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!