TZM-bl, HEK 293T/17 and HEK 293S/GnT1- cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) and gentamicin (50 μg/ml) in vented T-75 culture flasks (Corning-Costar). Cultures were incubated at 37°C in a humidified 5% CO2–95% air environment. Cell monolayers were split 1:10 at confluence by treatment with 0.25% trypsin, 1 mM EDTA.
T 75 culture flasks
Manufactured by Corning
Sourced in United Kingdom
The T-75 culture flask is a laboratory equipment designed for cell culture and tissue engineering applications. It provides a standardized and controlled environment for the growth and maintenance of cells in vitro. The flask has a surface area of 75 cm² and is made of clear, high-quality polystyrene material. It is suitable for a wide range of cell types and can be used in various research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
Gentamicin, by Merck Group (1 mentions)
293t 17 cells, by American Type Culture Collection (1 mentions)
Tissue culture plate, by Corning (1 mentions)
Fetal bovine serum (fbs), by EQT (1 mentions)
Brain derived neurotrophic factor (bdnf), by Abcam (1 mentions)
Dmem f12, by Abcam (1 mentions)
Dmem f12, by EQT (1 mentions)
4 protocols using t 75 culture flasks
1
Maintenance of TZM-bl, HEK 293T/17 and HEK 293S/GnT1- Cell Lines
2
Cultivation of Cell Lines for Research
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TZM-bl cells were obtained from the NIH AIDS Research and Reference Reagent Program (ARRRP, catalog no. 8129) as contributed by John Kappes and Xiaoyun Wu. 293T/17 cells were obtained from the American Type Culture Collection (catalog no. 11268). Both cell lines were maintained in Dulbecco’s modified Eagle’s medium (HyClone) containing 10% heat-inactivated fetal bovine serum (Hyclone) and 50 μg gentamicin/ml (Sigma) in vented T-75 culture flasks (Corning-Costar). The FreeStyle™ 293-F cell line was purchased from Invitrogen and was adapted to suspension culture in FreeStyle™ 293 Expression Medium. Cultures were incubated at 37 °C in a humidified 5% CO2–95% air environment with shaking at 225 rpm.
3
Maintenance of TZM-bl, HEK 293T/17 and HEK 293S/GnT1- Cell Lines
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TZM-bl, HEK 293T/17 and HEK 293S/GnT1- cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) and gentamicin (50 μg/ml) in vented T-75 culture flasks (Corning-Costar). Cultures were incubated at 37°C in a humidified 5% CO2–95% air environment. Cell monolayers were split 1:10 at confluence by treatment with 0.25% trypsin, 1 mM EDTA.
4
SH-SY5Y Cell Culture and Differentiation
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SH-SY5Y cells were maintained in T75 culture flasks (Costar, Cambridge, UK) in DMEM/F12 (Labtech, Heathfield, UK) supplemented with 10% (v/v) heat inactivated fetal bovine serum (Labtech, UK), penicillin (100 U/mL) and streptomycin (100 U/mL) at 37 °C in 5% CO2 and 95% humidity. When cells reached ~80% confluence, they were seeded into 96 well (viability assays) or 24 well (mRNA expression assays) tissue culture plates (Costar, Cambridge, UK) at a density of 5 × 105 cells/cm2 and incubated at 37 °C in 5% CO2 and 95% humidity for 24 h prior to experimentation, or differentiation followed by experimentation. Differentiation was achieved according to the method of de Medeiros et al. [32 (link)]; cells were incubated for 1 day in DMEM/F12 with 10% serum followed by 3 days in DMEM/F12 with 1% serum plus 10 μM Retinoic acid (RA) and then 3 days in DMEM/F12 with 1% serum plus 10 μM RA plus 50 ng/mL bone derived neurotrophic factor (BDNF) (Abcam, Cambridge, UK).
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