Radio immunoprecipitationassay ripa buffer

Manufactured by Solarbio

Sourced in China

RIPA buffer is a solution used in biochemistry and molecular biology for the lysis of cells and extraction of proteins. It is a common buffer used in techniques such as Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Enhanced chemiluminescence reagent, by Bio-Rad (1 mentions) Image lab, by Bio-Rad (1 mentions) β actin, by Abcam (1 mentions) Bicinchoninic acid assay, by Beyotime (1 mentions) Anti vegfa antibody, by Abcam (1 mentions)

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RIPA buffer (829 citations)

2 protocols using radio immunoprecipitationassay ripa buffer

Western Blot Analysis of NK Cell Proteins

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NK cells, NK92 cells, and tumor tissues were lysed in Radio Immunoprecipitation
Assay (RIPA) buffer (Beijing Solarbio Science and Technology, China). Protein
samples was separated by 10% SDS-PAGE and transferred to polyvinylidene fluoride
membrane (Millipore, Bedford, MA, USA). The membrane was incubated with primary
Abs against RUNX3 (1:1000; Cell Signaling Technology, Danvers, MA, USA), NCR1
(1:500; Abcam, Cambridge, MA, USA) at 4°C overnight, and then incubated with
HRP-conjugated secondary Ab (Abcam, USA) at room temperature for 1 h. Bands were
visualized by an enhanced chemiluminescence reagent (Bio-Rad), and band
intensities were quantified using image software Image Lab (Bio-Rad). β-Actin
(Abcam) was used as an internal control.

Fang P., Xiang L., Chen W., Li S., Huang S., Li J., Zhuge L., Jin L., Feng W., Chen Y, & Pan C. (2019). LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3. Innate Immunity, 25(2), 99-109.

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Western Blot Analysis of VEGF Protein

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VEGF protein expression was detected using Western blotting. Proteins were extracted using Radioimmunoprecipitation assay (RIPA) buffer (Solarbio, China), which contained protease inhibitors. The Bicinchoninic acid assay (Beyotime, China) method was used to quantify the concentration of the collected proteins. The samples were electrophoresed on polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane. The membrane was blocked with 5% skim milk for 2h. Subsequently, the corresponding anti-VEGFA antibody (1:1000, Abcam, UK) was incubated at 4°C for 12 h. After washing with Tris-buffered saline with Tween solution (TBST), the membranes were incubated with the corresponding secondary antibody (ZSGB-BIO, China) for 1 h at room temperature. Images were visualized using a multicolor fluorescence imaging system (Amersham, UK). Grey level analysis was performed using the ImageJ software.

Shi S., Gong Y., Hu H., Peng S, & Liu J. (2023). Topical Dihydroartemisinin Improves Wound Healing in Diabetic Mice. Journal of plastic surgery and hand surgery, 58.

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