For whole liver tissue analysis, representative pieces of liver were placed into Trizol™ (Ambion, Life Technologies) and homogenized. RNA was isolated from using the RNeasy mini-kit (Qiagen) as per the manufacturer’s instructions. For analysis of sorted cells, Trizol™ was used to lyse the cells and RNA was isolated using either the RNeasy mini-kit (Qiagen) or RNeasy micro-kit (Qiagen) per the manufacturer’s instructions. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed using the SYBR Green or Taqman qPCR kit (Applied Biosystems) and normalized to the housekeeping gene Gapdh by comparative Ct.
Sybr green or taqman qpcr kit
Manufactured by Thermo Fisher Scientific
SYBR Green or TaqMan qPCR kits are specialized reagents used for quantitative real-time PCR (qPCR) analysis. These kits contain the necessary components, including DNA polymerase, fluorescent dyes, and buffers, to detect and quantify target DNA sequences in a real-time PCR reaction. The core function of these kits is to enable the sensitive and accurate measurement of gene expression levels or the detection of specific DNA targets in a sample.
Automatically generated - may contain errors
2 protocols using sybr green or taqman qpcr kit
1
Transcriptional Analysis of Liver Tissue
2
Transcriptional Analysis of Liver Tissue
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For whole liver tissue analysis, representative pieces of liver were placed into Trizol™ (Ambion, Life Technologies) and homogenized. RNA was isolated from using the RNeasy mini-kit (Qiagen) as per the manufacturer’s instructions. For analysis of sorted cells, Trizol™ was used to lyse the cells and RNA was isolated using either the RNeasy mini-kit (Qiagen) or RNeasy micro-kit (Qiagen) per the manufacturer’s instructions. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed using the SYBR Green or Taqman qPCR kit (Applied Biosystems) and normalized to the housekeeping gene Gapdh by comparative Ct.
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