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GB11064 is a laboratory equipment designed for cell culturing purposes. It serves as an incubator to provide a controlled environment for cell growth and maintenance.

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Lab products found in correlation

Gb11067, by Wuhan Servicebio Technology (1 mentions) Gb21303, by Wuhan Servicebio Technology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) A1 spectral confocal microscope, by Nikon (1 mentions) Cy3 goat anti rabbit, by Wuhan Servicebio Technology (1 mentions) Gb113109, by Wuhan Servicebio Technology (1 mentions) Gb13014, by Wuhan Servicebio Technology (1 mentions) Gb12068, by Wuhan Servicebio Technology (1 mentions) Gb11059, by Wuhan Servicebio Technology (1 mentions) Gb13445, by Wuhan Servicebio Technology (1 mentions) Anti cd3, by Wuhan Servicebio Technology (1 mentions) Gb13585, by Wuhan Servicebio Technology (1 mentions) Anti cd11c, by Wuhan Servicebio Technology (1 mentions) Alexa fluor 488 goat anti mouse, by Wuhan Servicebio Technology (1 mentions) Anti ki67, by Wuhan Servicebio Technology (1 mentions) Gb21301, by Wuhan Servicebio Technology (1 mentions) Gb22302, by Wuhan Servicebio Technology (1 mentions) Gb121141, by Wuhan Servicebio Technology (1 mentions) Gb25301, by Wuhan Servicebio Technology (1 mentions) Gb11068 1, by Wuhan Servicebio Technology (1 mentions)

Spelling variants of this product

GB11064-1 (2 citations)

3 protocols using gb11064

1

Immunohistochemical Detection of CD4 and CD68

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Lung tissue sections were incubated with the primary antibodies anti-CD4 (Servicebio, GB11064) at 1:1,000 and anti-CD68 (Servicebio, GB11067) at 1:300, and then incubated with the HRP-goat anti-rabbit secondary antibody (Servicebio, GB21303) at 1:200 dilution. Color was developed by DAB. The tissue sections were observed under a microscope.
Song J., Zhao M., Li Q., Lu L., Zhou Y., Zhang Y., Chen T., Tang D., Zhou N., Yin C., Weng D, & Li H. (2019). IL-17A Can Promote Propionibacterium acnes-Induced Sarcoidosis-Like Granulomatosis in Mice. Frontiers in Immunology, 10, 1923.
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2

Immunohistochemical Analysis of Tumor Microenvironment

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After fixation in 4 % PFA, the tumor tissues and lymph nodes were dehydrated in a 20 % sucrose solution, embedded in paraffin, and subsequently cut into slices (5 μm). After incubation with 5 % BSA for 1 h, primary antibodies were added (anti-CD3, 1:500, GB13014; anti-CD4, 1:500, GB11064; anti-CD8, 1:2000, GB12068; anti-Foxp3, 1:500, GB13445; anti-CD68, 1:200, GB113109; anti-Ki67, 1:600, GB121141; anti-CD86, 1:300, GB13585; anti-CD11c, 1:300 GB11059, all from Servicebio, Wuhan, China) at 4 °C in a dark, wet box overnight. After extensive washing using FACS buffer, sections were incubated with secondary antibodies (FITC-goat anti-rat, 1:200, GB22302; CY3-goat anti-rabbit, 1:500, GB21301; Alexa Fluor 488-goat anti-mouse, 1:500, GB25301, all from Servicebio) for 1 h at 25 °C. Nuclei were stained with Hoechst 33342 (Thermo Fisher Scientific, Waltham, MA, USA). The stained sections were observed using a Nikon A1 spectral confocal microscope (Nikon, Tokyo, Japan).
Xing Y., Zhang F., Yang T., Yin C., Yang A., Yan B, & Zhao J. (2024). Augmented antitumor immune responses of HER2-targeted pyroptotic induction by long-lasting recombinant immunopyroptotins. Heliyon, 10(9), e30444.
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3

Immunohistochemical Analysis of Tumor Infiltration

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Tumor tissues and spleens resected from mice were fixed in 4% paraformaldehyde and then embedded in paraffin for subsequent sectioning. Each sample was cut into 4 μm sections and then incubated using mouse antihuman CD4 antibody (1:100 dilution; rabbit no. GB11068-1, SERVICEBIO) and anti-CD8 antibody (1:100 dilution; rabbit no. GB11064, SERVICEBIO) overnight at 4°C to analyze spleen infiltration, which was then further incubated using a rabbit antimouse antibody at room temperature for 2 h (1:1,000 dilution; rabbit no. SP-9001, Beijing Zhongshan Jinqiao Biological Company). Thereafter, the sections were stained using diaminobenzidine (K135925C, Beijing Zhongshan Jinqiao Biological Company) and hematoxylin. For approximately 2 min, the sections were stained using a DAB color development kit.
Luo X, & Zeng M. (2024). Combination low-dose cyclophosphamide with check-point blockade and ionizing radiation promote an abscopal effect in mouse models of melanoma. Journal of cancer research and therapeutics, 20(2).
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