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Phrl tk renella luciferase control vector

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The PhRL-TK-Renella luciferase control vector is a plasmid that contains the Renilla luciferase gene under the control of the Herpes Simplex Virus Thymidine Kinase (HSV-TK) promoter. This vector can be used as a control for luciferase reporter assays.

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Lab products found in correlation

Passive lysis buffer (plb), by Promega (2 mentions) Dual luciferase assay kit, by Promega (1 mentions) Nano glo dual luciferase assay system, by Promega (1 mentions)

2 protocols using phrl tk renella luciferase control vector

1

Dual-Luciferase Assay for IFNB1 Promoter

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Indicated cells were seeded in 12-well plates for 12 h and co-transfected with pGL3-IFNB1 reporter plasmid and a phRL-TK-Renella luciferase control vector (Addgene). After 24 h, indicated reagents were added, the cells were lysed in 1× passive lysis buffer (Promega) for 24 h, and the luciferase activities were determined using the Dual-Luciferase® Assay kit (Promega). Firefly luciferase activity was normalized to Renilla luciferase activity for each sample.
Ma Z., Li Z., Mao Y., Ye J., Liu Z., Wang Y., Wei C., Cui J., Liu Z, & Liang X. (2023). AhR diminishes the efficacy of chemotherapy via suppressing STING dependent type-I interferon in bladder cancer. Nature Communications, 14, 5415.
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2

Dual-Luciferase Assay for Transcriptional Regulation

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SUNE1, HONE1, NP69, or N2‐Tert cells (4 × 104) were seeded in 24‐well plates for 12 h and co‐transfected with plasmids encoding empty vector or FLAG‐ZNF582 or shZNF582, along with the pGL3‐Nectin‐3 or NRXN3 reporter plasmid (100 ng) and a phRL‐TK‐Renella luciferase control vector (Addgene, 10 ng). After 24 h, the cells were lysed in 1 × passive lysis buffer (Promega), and the luciferase activities were determined using the Nano‐Glo® Dual‐Luciferase® Assay System (Promega). Afterwards, the firefly luciferase activity was normalized through the Renella luciferase activity, and the results were presented as the fold change relative to the activity in the empty vector‐transfected cells.
Zhao Y., Hong X., Li K., Li Y., Li Y., He S., Zhang P., Li J., Li Q., Liang Y., Chen Y., Ma J., Liu N, & Chen Y. (2020). ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin‐3 and NRXN3. Cancer Communications, 40(12), 721-737.
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