All fly stocks were maintained at 25 °C on standard medium unless otherwise stated. The following stains were used in this study: w1118 (BDSC BL6326), Act-Gal4 (BDSC BL3954), Da-Gal4 (BDSC BL55851), Ub-Gal4 (BDSC BL32551), Caudal (Cad)-Gal4 (BDSC BL3042), R4-Gal4 (BDSC BL33832), ElaV-Gal4 (BDSC BL458), Mhc-Gal4 (BDSC BL38464), Hsp70-Gal4 (BDSC BL2077), Escargot-Gal4 (Kyoto DGRC 114-042), Prospero-Gal4 (BDSC 80572), UAS-dSLC5A5RNAi (BDSC BL63568), UAS-dSLC5A5RNAi (VDRC 40650), and UAS-shibireRNAi (BDSC BL28513). The MyoIA-Gal4 fly line is a gift from Dr. Bruce Edgar (University of Utah, Salt Lake City, UT, USA). The dSLC5A5-FLAG clone (UFO11244) was obtained from the Drosophila Genomics Resource Center (DGRC) and contains full-length dSLC5A5 cDNA with a C-terminus FLAG HA tag in a pUAST vector. UAS-dSLC5A5-FLAG transgenic flies were generated by BestGene Inc. (Chino Hills, CA, USA). For each cross, the Gal4 or UAS-RNAi progenies derived from the cross are used as controls.
Act gal4
Manufactured by BD
Act-Gal4 is a genetic tool used in biological research. It functions as a transcriptional activator, driving the expression of target genes in cells or organisms. The core purpose of Act-Gal4 is to enable the controlled and targeted regulation of gene expression for experimental investigations.
Automatically generated - may contain errors
2 protocols using act gal4
1
Drosophila Genetic Toolkit: Diverse Strains
2
Drosophila Genetics: Characterizing ski3 Mutants
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Drosophila stocks were raised at 25 °C under a 12-h light and 12-h dark cycle, using a standard cornmeal-sugar-agar medium containing propionic acid and n-butyl p-hydroxybenzoate as mold inhibitors. To characterize ski3 mutants, w 1118 ;PBac {w +mC =WH}CG8777 f03251 /CyO, act-GFP (BDSC #18632; Bloomington, IN, USA) was backcrossed to our laboratory strain w 1118 (used as a control) for at least six generations prior to use in the experiments. arm-gal4 (BDSC #1561) and UAS-ski3-IR (NIG-FLY #8777R-1; Mishima, Japan) were used to knock down ski3. Mosaic clones were generated with an FLP/ FRT-mediated recombination system using the following stocks: DGRC #108449: w 1118 ;P{w +mW.hs =FRT(w hs )}G13P {w +mC =Ubi-GFP.nls}2R1P{w +mC =Ubi-GFP.nls}2R2, DGRC #141404: w*;P{w +mW.hs =FRT(w hs )}G13, y*w*;P{ry +t7.2 = neoFRT}40A P{w +mW.hs =FRT(w hs )}G13 cn 1 PBac{SAstop DsRed}LL04977 bw 1 /CyO, S* bw 1 , BDSC #6: P{ry +t7.2 = hsFLP}1, w 1118 ;Adv 1 /CyO. Heat shock was applied for 1 h at 37 °C to embryos at 0-6 h of age [13] . act-Gal4 (BDSC #3954) was used as a driver of UAS transgenes.
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