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10xuas myr gfp

Manufactured by BD

The 10xUAS-myr::GFP is a genetic construct that produces a myristoylated green fluorescent protein (GFP) under the control of the UAS (Upstream Activating Sequence) promoter. This construct is commonly used in various research applications to study gene expression and cellular localization.

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2 protocols using 10xuas myr gfp

1

Drosophila Genetic Toolkit Protocols

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Flies were kept at 25 °C 12-hour light/dark cycles on a standard cornmeal medium. 10xUAS-myr::GFP (BDSC #32197), UAS-RedStinger (#8546), Tret1–1-Gal4 (#67471), Vmat-Gal4 (#66806), rost::LacZ (#12042), CG3036::GFP (#50804), b-Gal4 (#76724), DAT-Gal4 (#84622), CG31663-Gal4 (#76708), ome-Gal4 (#76166), Canton-S (#64349), Hand-Gal4 (#66795), CG4288-Gal4::GFP (#29208), alphaTub85E::GFP (#60267), Npc2b-Gal4::GFP (#24694), Adgf-D-Gal4 (#77799), R25A01-Gal4 (#49102), UASCD8:GFP (from Bloomington), Cyp4g15-Gal4 (#39103), GstT4-Gal4 (#77777), nkd::LacZ (#25111), R94A08-Gal4 (#40673), UAS-GFP.nls (#4776), UAS-sfGFPnlsPEST-2A-B2ase – this is an unpublished line developed by Dr. Benjamin Choi (Desplan lab), consisting of a destabilized nuclear-localized GFP (Table Fly Genotypes).
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2

Validating Tm9 and T4/T5 Neuron Subtypes

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The following transgenic lines were used for validation of regional subtypes of Tm9 and T4/T5 neurons: 24C08-LexA (Tm9, BDSC #62012), 23G12-LexA (T4/T5, BDSC #65044), Wnt10-TG4 (gift from Hugo Bellen, CR01661), CG15537-TG4 (gift from Hugo Bellen, CR02107), LexAop-myr::tdTomato (Zipursky laboratory), 10XUAS-myr::GFP (BDSC #32197).
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