CT26 and A20 cells were purchased from ATCC and cultured in RPMI-1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. A20 cells were grown in the same medium and additionally supplemented with 0.01% 2-mercaptoethanol. Mammalian cells were grown inside a 37°C tissue culture incubator (ThermoFisher Scientific) maintained at 5% CO2.
37 c tissue culture incubator
Manufactured by Thermo Fisher Scientific
The 37°C tissue culture incubator is a piece of laboratory equipment designed to maintain a stable temperature of 37°C, which is the optimal temperature for the growth and maintenance of many types of cells and tissues in a controlled environment.
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Lab products found in correlation
2 protocols using 37 c tissue culture incubator
1
Culturing CT26 and A20 Cells
2
Enrichment of Giant Cell Tumor Stroma
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To enrich for the stromal compartment of the GCTB, we minced tissues in culture media (DMEM, 10% fetal bovine serum and 1% penicillin/streptomycin (HyClone)), supplemented with 1.5 mg/mL collagenase B (Roche Diagnostics), prior to incubating 1 hour at 37 °C. The disintegrated tissues were collected and spun down, and the pellets were washed twice with PBS. Resuspended pellets were incubated in cell culture media and incubated for 24 hours in a 37 °C tissue culture incubator (Thermo Fisher Scientific, 5% CO2, 80% humidity). Attached cells were treated with 0.05% trypsin-EDTA for 2 minutes to remove loosely attached cells, followed by 0.25% trypsin-EDTA for 5–8 minutes at 37 °C to remove the remaining cells (stromal compartment).
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